FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514590
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTGGAGTGGGTG26680.5184710157601197No Hit
GTATATAGTAGTCCGTGAAGGTATATCCAGAAGCCTTGCAGGAGACCTTC11490.22328455663732294No Hit
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTGGAGTGGGTGG9880.19199751258283293No Hit
GTCTACATGGAACTGAGCGGACTGGGATCTGACGACACGGCCGTATATTT8690.16887230610777512No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8440.1640140694533512No Hit
CTACTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTGGAGTGGG8110.15760119706951164No Hit
GTCTGAGACTGAGGTGAAGAAGCCTGGGGCCTCAATGAAGGTCTCCTGCA8050.1564352202724499No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA7950.15449192561068037No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT7880.15313161934744166No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT7740.15041100682096428No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC7240.14069453351211644No Hit
GTGTATATAGTAGTCCGTGAAGGTATATCCAGAAGCCTTGCAGGAGACCT7120.13836257991799294No Hit
ACATGGAACTGAGCGGACTGGGATCTGACGACACGGCCGTATATTTCTGT7100.13797392098563904No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG7090.13777959151946212No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT6770.1315610486017995No Hit
CCCCTAATGCTGGCACAAACTATCCACAGAAGTTTCAGGGCAGGGTCTCC6560.1274801298120834No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT6260.12165024582677471No Hit
ATATACGGCCGTGTCGTCAGATCCCAGTCCGCTCAGTTCCATGTAGACTG6160.11970695116500515No Hit
CATGTAGACTGTGTTGATGGACGTGTCTGTGGTCATGGAGACCCTGCCCT6160.11970695116500515No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC6150.11951262169882819No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCCAAACACCGCCA5990.1164033502399969No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5760.11193377251792688Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG5650.10979614838998036No Hit
GGGTGGGACTGATCACCCCTAATGCTGGCACAAACTATCCACAGAAGTTT5640.10960181892380341No Hit
ATATAGTAGTCCGTGAAGGTATATCCAGAAGCCTTGCAGGAGACCTTCAT5590.10863017159291864No Hit
CCTCTAGAGAATCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACCTG5580.10843584212674166No Hit
GTTCCATGTAGACTGTGTTGATGGACGTGTCTGTGGTCATGGAGACCCTG5470.10629821799879514No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG5310.10318894653996386No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)