FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514590
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTGGAGTGGGTG20540.3991527235274685No Hit
GTATATAGTAGTCCGTGAAGGTATATCCAGAAGCCTTGCAGGAGACCTTC11480.22309022717114596No Hit
CTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTGGAGTGGGTGG8340.16207077479158163No Hit
GTCTACATGGAACTGAGCGGACTGGGATCTGACGACACGGCCGTATATTT7920.1539089372121495No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT7620.14807905322684078No Hit
GTGTATATAGTAGTCCGTGAAGGTATATCCAGAAGCCTTGCAGGAGACCT7010.13622495579004643No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA6940.13486464952680774No Hit
CTACTATATACACTGGGTGCGACAGGCCCCTGGACAAGGGCTGGAGTGGG6930.1346703200606308No Hit
GTCTGAGACTGAGGTGAAGAAGCCTGGGGCCTCAATGAAGGTCTCCTGCA6880.133698672729746No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT6820.13253269593268427No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC6590.12806311821061428No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT6570.12767445927826035No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG6030.11718066810470472No Hit
ACATGGAACTGAGCGGACTGGGATCTGACGACACGGCCGTATATTTCTGT5870.11407139664587343No Hit
CTCCTGGACCCCTGCCCGGTTCTCAGGCTCCCTCCTTGGGGGCAAAGCTG5670.11018480732233428No Hit
CCCCTAATGCTGGCACAAACTATCCACAGAAGTTTCAGGGCAGGGTCTCC5640.10960181892380341No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC5480.10649254746497212No Hit
ATATACGGCCGTGTCGTCAGATCCCAGTCCGCTCAGTTCCATGTAGACTG5390.1047435822693795No Hit
ATATAGTAGTCCGTGAAGGTATATCCAGAAGCCTTGCAGGAGACCTTCAT5250.1020229697429021No Hit
GTTCCATGTAGACTGTGTTGATGGACGTGTCTGTGGTCATGGAGACCCTG5240.10182864027672515No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAT11200.034.1026651
AAGCGCT1905.808033E-934.0967259
CTATATA11650.033.9829062
TATATAC13200.033.8097153
GTACACG3350.032.2307365
TACTGAC1352.2477354E-531.991997
TCAACGC14450.030.3868544
TCTACAC3800.030.30823
ATACACT13550.029.7490086
ATCAACG14900.029.469133
CCTAGGT6000.028.7927912
CTAGGTC6000.028.7927913
GAATACG1255.3711445E-428.792799
CAACGCA15650.028.5168195
ATATACA15400.028.5123424
TACACTG13350.028.0379247
GTATCAA16650.027.7010481
TACACGG3950.027.3349276
TATCAAC16100.027.2726752
GCCCATA1607.10637E-527.0247521
Produced by FastQC (version 0.11.5)