FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890143
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCATGTAGG29170.32770015604234376No Hit
ATCCTAGAGGGGTACGCCAATCTCTCGCACAGTAATACACGGCCGTGTCG18320.20580962834061495No Hit
GTGTATTACTGTGCGAGAGATTGGCGTACCCCTCTAGGATATAGCAGTGG16400.1842400603049173No Hit
GCATAATAGTTACCCCCCCACCTTCGGCCAAGGGACACGACTGGAGATTA16240.1824425963019425No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16010.1798587417976662No Hit
GCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTA15720.1766008382922744No Hit
GCTATATCCTAGAGGGGTACGCCAATCTCTCGCACAGTAATACACGGCCG15450.1735676177872544No Hit
GTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA14980.16828756727851593No Hit
CTATTATGCTGTAGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT13410.15064995174932566No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12640.14199965623500943No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12310.1382923867288739No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12240.13750599622757242No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC12240.13750599622757242No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT11880.1334617022208791No Hit
TCCTAGAGGGGTACGCCAATCTCTCGCACAGTAATACACGGCCGTGTCGT11590.1302037987154873No Hit
CTCTAGGATATAGCAGTGGCTGGGAGGATTACTACTACTACTACGGTATG11250.12638418770916585No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11090.12458672370619103No Hit
CTCCCAGGTTCAGCTGGTGCAGTCTGGAGCTGAGGTGAAGAAGCCTGGGG10370.11649813569280443No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10160.11413896418889999No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGG10110.11357725668797035No Hit
CCATGTAGGCTGTGCTCGTGGATGTGTCTGTGGTCATGGTGACTCTGCCC10090.11335257368759852No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9990.11222915868573927No Hit
CCGTAGTAGTAGTAGTAATCCTCCCAGCCACTGCTATATCCTAGAGGGGT9890.11110574368388001No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9650.10840954767941781No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9530.1070614496771867No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9490.106612083676443No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9440.10605037617551338No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGATGGATCAGCGCTTACAATGGT9230.10369120467160894No Hit
CATGTAGGCTGTGCTCGTGGATGTGTCTGTGGTCATGGTGACTCTGCCCT9010.1012196916675186No Hit
GGTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGG8970.1007703256667749No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8940.10043330116621711No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG11200.048.8629383
TCAACGC11350.048.2171754
GTATCAA12250.048.215191
CAACGCA12200.044.857785
TATCAAC13300.041.6938442
AACGCAG13400.041.3710676
CGCAGAG14650.037.349678
TACACCG1204.2226363E-430.003565
GTACTAA1856.117536E-627.2541371
ACGCAGA20100.027.2225197
TAATAGT12650.026.7541624
GGCAGCG4350.026.4814367
ATAATAG13450.026.2335973
GTACACG6500.025.4799465
GTACATG21350.025.3028551
ACATGGG21250.025.0759143
TCTACAC6500.024.3721223
CATAATA13500.024.0055472
AATAGTT12700.023.8138485
GTATATA5150.023.7764931
Produced by FastQC (version 0.11.5)