FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2956777
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAAGGGTTTGGAGTGGTTCACAGTCGTCGGATACTACTTTGACTTCT60130.20336332432239565No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT58290.19714033219278962No Hit
CCCTTAGACCGCGCACAGTAATAGACACCCGAGTCTTCAGCTCTCAGGCT50880.17207926062736556No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40970.1385630367119333No Hit
GTAATAGACACCCGAGTCTTCAGCTCTCAGGCTGCTCATTTGAAAATAGA38330.1296343958303247No Hit
GTCTATTACTGTGCGCGGTCTAAGGGTTTGGAGTGGTTCACAGTCGTCGG37880.12811246840732327No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC34630.11712077035231268No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31700.10721133179810315No Hit
GTATATTGCTGACAGTAATAAACTGCAGAATCCTCAGACTGCAGGCTGCT31200.10552030132810152No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGCGCCACCCTCTCTT31150.10535119828110134No Hit
GCAATATACTCACTGGCCTCCGTACACTTTTGGCCAGGGGACCAAGGTGC29950.10129272515309745No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)