FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767226
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCTTCAGGTCTCAGGCTGTTCATATGAAGATAGA31510.4107003672972501No Hit
GTATAATAACTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA23120.3013453662936345No Hit
GTGTATTACTGTGCGAGAGCCGGGGTCCAACACTCTGATACTGGTGGTTA19530.2545534171156869No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA18030.23500246342016565No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17750.23135295206366835No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC16240.21167165867684357No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT15700.20463331534645593No Hit
GTATCAGAGTGTTGGACCCCGGCTCTCGCACAGTAATACACGGCCGTGTC15600.20332991843342116No Hit
CTCCTAGGGCGACCGAATAACCACCAGTATCAGAGTGTTGGACCCCGGCT15420.2009838039899586No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15300.19941972769431693No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15020.19577021633781963No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14660.19107798745089452No Hit
CCCTAGGAGCTTTTGATTTCTGGGGCCAAGGGACAATGGTCACCGTCTCT14500.18899255239003893No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT14490.18886221269873543No Hit
CTCTGATACTGGTGGTTATTCGGTCGCCCTAGGAGCTTTTGATTTCTGGG13980.1822148884422582No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC13820.18012945338140263No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13750.17921707554227828No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12520.16318529351195085No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12410.16175155690761261No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12130.15810204555111532No Hit
CTACATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCT11330.14767487024683731No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10840.14128822537296704No Hit
CTTCCCAGACTCCGTGAAGGACCGATTCACCATCTCCAGACACAGTTCCA10750.14011516815123573No Hit
CTCCTGTGCAGCCTCTGGGTTCACCGTCAGTAACCACTACATGACCTGGG10350.13490158049909673No Hit
CATATGAACAGCCTGAGACCTGAAGACACGGCCGTGTATTACTGTGCGAG9930.1294273134643508No Hit
GTTCATATGAAGATAGAGGGTGTTGCTGGAACTGTGTCTGGAGATGGTGA9870.12864527531652994No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9870.12864527531652994No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC9740.12695085932958478No Hit
CTCCAGACACAGTTCCAGCAACACCCTCTATCTTCATATGAACAGCCTGA9570.12473508457742567No Hit
GATAGAGGGTGTTGCTGGAACTGTGTCTGGAGATGGTGAATCGGTCCTTC9450.12317100828178398No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT9390.12238897013396313No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGGTTCACCGTCAGTAACCACTAC9320.12147659229483881No Hit
GTATTACTGTGCGAGAGCCGGGGTCCAACACTCTGATACTGGTGGTTATT9200.11991251599919711No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9110.11873945877746583No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8960.1167843634079137No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8950.11665402371661023No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8850.11535062680357547No Hit
CCCTCTATCTTCATATGAACAGCCTGAGACCTGAAGACACGGCCGTGTAT8820.11495960772966506No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8750.11404722989054071No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8640.1126134932862025No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG8540.11131009637316774No Hit
GTCTGGGAAGTATGTGTCACCACCGCTATAAATAATCGAGACCCACTCCA8510.11091907729925733No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT8340.10870330254709826No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8320.1084426231644913No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA8180.10661786748624265No Hit
GTGTCTGGAGATGGTGAATCGGTCCTTCACGGAGTCTGGGAAGTATGTGT8170.10648752779493917No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC8140.10609650872102874No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC8130.10596616902972526No Hit
GTCTGGAGGAGGCTTGGTCCAGCCGGGGGGGTCCCTGAGACTCTCCTGTG8000.1042717530427801No Hit
CAGTAATACACGGCCGTGTCTTCAGGTCTCAGGCTGTTCATATGAAGATA7980.10401107366017313No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG7760.10114360045149669No Hit
GAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGGTTTTCCTTGT7710.1004919019949793No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)