FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767226
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCTTCAGGTCTCAGGCTGTTCATATGAAGATAGA32160.41917244723197594No Hit
GTATAATAACTGGCCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA24600.32063564060654876No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21710.2829674698198445No Hit
GTGTATTACTGTGCGAGAGCCGGGGTCCAACACTCTGATACTGGTGGTTA20250.2639378748895371No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16280.21219301744205749No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC16100.20984690299859493No Hit
CTCCTAGGGCGACCGAATAACCACCAGTATCAGAGTGTTGGACCCCGGCT15650.20398161688993857No Hit
GTATCAGAGTGTTGGACCCCGGCTCTCGCACAGTAATACACGGCCGTGTC15380.20046244522474474No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT15280.19915904831171No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15210.19824667047258568No Hit
CCCTAGGAGCTTTTGATTTCTGGGGCCAAGGGACAATGGTCACCGTCTCT14680.19133866683350148No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC14090.18364862504659643No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14010.18260590751616865No Hit
CTCTGATACTGGTGGTTATTCGGTCGCCCTAGGAGCTTTTGATTTCTGGG13900.1811721709118304No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13610.17739231986402965No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13580.1770013007901192No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT13550.1766102817162088No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12320.16057849968588134No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11660.15197608005985197No Hit
CTTCCCAGACTCCGTGAAGGACCGATTCACCATCTCCAGACACAGTTCCA11380.1483265687033547No Hit
CTACATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTCT11320.14754453055553382No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10780.14050618722514618No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10740.1399848284599323No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10650.13881177123820101No Hit
CTCCTGTGCAGCCTCTGGGTTCACCGTCAGTAACCACTACATGACCTGGG10600.13816007278168363No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10540.13737803463386278No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10370.1351622598817037No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC10280.1339892026599724No Hit
GTTCATATGAAGATAGAGGGTGTTGCTGGAACTGTGTCTGGAGATGGTGA10020.13060037068608207No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGGTTCACCGTCAGTAACCACTAC9840.12825425624261952No Hit
CTCCAGACACAGTTCCAGCAACACCCTCTATCTTCATATGAACAGCCTGA9700.12642950056437088No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9270.12082489383832143No Hit
CATATGAACAGCCTGAGACCTGAAGACACGGCCGTGTATTACTGTGCGAG9180.11965183661659015No Hit
GTATTACTGTGCGAGAGCCGGGGTCCAACACTCTGATACTGGTGGTTATT9000.11730572217312761No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8940.11652368402530675No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC8860.11548096649487895No Hit
GATAGAGGGTGTTGCTGGAACTGTGTCTGGAGATGGTGAATCGGTCCTTC8850.11535062680357547No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT8820.11495960772966506No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG8810.11482926803836158No Hit
CCCTCTATCTTCATATGAACAGCCTGAGACCTGAAGACACGGCCGTGTAT8810.11482926803836158No Hit
GTCTGGGAAGTATGTGTCACCACCGCTATAAATAATCGAGACCCACTCCA8800.1146989283470581No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT8740.11391689019923726No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8710.11352587112532682No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC8440.11000669946013299No Hit
CAGTAATACACGGCCGTGTCTTCAGGTCTCAGGCTGTTCATATGAAGATA8300.10818194378188434No Hit
GTGTCTGGAGATGGTGAATCGGTCCTTCACGGAGTCTGGGAAGTATGTGT8230.10726956594276002No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8220.10713922625145654No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA8110.10570548964711833No Hit
GTCTGGAGGAGGCTTGGTCCAGCCGGGGGGGTCCCTGAGACTCTCCTGTG7970.10388073396886968No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC7960.1037503942775662No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT7780.10140427983410365No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG7680.10010088292106889No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC9500.058.388012
GTATCAA10100.056.3495671
TCAACGC9900.055.2904244
ATCAACG10100.054.1991043
CAACGCA10450.052.3804055
AACGCAG11250.048.6333736
CGCAGAG11850.046.1709258
GGCAGCG4550.037.9728437
GCAGAGT14600.037.4718979
GAATACG1708.3236955E-833.8755269
GGTATCA5000.033.139251
GTACATG16950.031.45191
ACATGGG18000.031.2120253
GCAGCGC5500.030.104998
ACGCAGA18750.029.947927
GTTAGGG1703.1677664E-629.6584873
TACATGG18600.029.4345362
GTGCTAG3251.8189894E-1228.816741
ATTAGGC1255.353614E-428.8111023
TAACTGG15550.028.2405247
Produced by FastQC (version 0.11.5)