FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2163472
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCGTTAGGTCTCAGGCTGCTCATTTGCAGATACA81040.37458307757160714No Hit
GTATATTACTGTGTCAAAGATGGGTGGGCGACGTGGGGCCAGGGAACCCT74350.34366056043249No Hit
GTATAATAGTTATTCGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAAC73490.33968546854315657No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCT61570.28458884607704654No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT49950.23087888357233186No Hit
ATTAAATACTATGCAGACTCCGTGCTAGACCGATTCACCATCTCCAGAGA46490.21488607201757176No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG44880.20744433022474984No Hit
ATATACAGCCGTGTCGTTAGGTCTCAGGCTGCTCATTTGCAGATACAGCG42740.19755282250012943No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40790.18853953275105942No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT40450.1869679847948113No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT39740.18368622288617556No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA39060.18054312697367933No Hit
GTGCTAGACCGATTCACCATCTCCAGAGACGATTCCAAGAACACGCTGTA37770.1745804891396792No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC36790.1700507332657876No Hit
GCTGTATATTACTGTGTCAAAGATGGGTGGGCGACGTGGGGCCAGGGAAC36390.16820185331726042No Hit
GTATTTAATACTTACATCATATGATATGACTGCCACCCACTCCAGCCCCT35630.16468898141505875No Hit
GTCTAGCACGGAGTCTGCATAGTATTTAATACTTACATCATATGATATGA34980.1616845514987021No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC34500.15946589556046947No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCGCA31450.1453681859529497No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG30650.14167042605589533No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCG30280.1399602121035077No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA29630.13695578218715102No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC29280.13533801223218975No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC28550.13196380632612764No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT27500.12711049646124378No Hit
CAGTATAATAGTTATTCGACGTTCGGCCAAGGGACCAGGGTGGAAATCAA26680.12332029256676305No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG26500.12248829658992583No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG26340.12174874461051495No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25350.11717276673791016No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT25070.11587855077394116No Hit
ATATTACTGTGTCAAAGATGGGTGGGCGACGTGGGGCCAGGGAACCCTGG24530.11338256284342946No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG23810.11005457893608053No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23790.10996213493865417No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC23700.10954613695023555No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG23460.10843680898111924No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23430.10829814298497971No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23040.1064954850351657No Hit
GTCTCAGGCTGCTCATTTGCAGATACAGCGTGTTCTTGGAATCGTCTCTG22790.10533993506733622No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT22710.10497015907763077No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA22100.10215061715612682No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG21990.10164217517028185No Hit
ATACTATGCAGACTCCGTGCTAGACCGATTCACCATCTCCAGAGACGATT21930.10136484317800276No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTATGCTATGCACTGGG21690.10025551520888645No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37650.082.0771261
ATCAACG44300.067.126723
TCAACGC44650.066.439264
CAACGCA47250.062.783355
TATCAAC48350.061.9492262
AACGCAG48800.060.7892076
CGCAGAG57700.051.411528
GCAGAGT66550.044.4664889
ACGCAGA68950.043.1275567
GTACATG62200.036.2479741
TACATGG61900.035.4771082
ACATGGG63550.033.9902843
CATGGGA31550.032.863384
CAGCGCT13300.031.9403329
GTACACG19550.031.3055425
GTAATAT41150.030.9836961
GTATGTA25300.029.6102851
CAGCCGT42650.029.3744169
AATATAC42750.029.1379573
TACACGG21050.029.0747436
Produced by FastQC (version 0.11.5)