FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2163472
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCGTTAGGTCTCAGGCTGCTCATTTGCAGATACA77640.35886759800912604No Hit
GTATAATAGTTATTCGACGTTCGGCCAAGGGACCAGGGTGGAAATCAAAC67510.3120447133126752No Hit
GTATATTACTGTGTCAAAGATGGGTGGGCGACGTGGGGCCAGGGAACCCT63600.29397191181582194No Hit
CTATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCT61420.28389551609634883No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT45940.21234386208834688No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG41950.19390128460178824No Hit
ATATACAGCCGTGTCGTTAGGTCTCAGGCTGCTCATTTGCAGATACAGCG40420.18682931879867176No Hit
ATTAAATACTATGCAGACTCCGTGCTAGACCGATTCACCATCTCCAGAGA40000.18488799485271823No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT39270.18151378894665612No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37660.1740720471538342No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT37140.17166850322074886No Hit
GTGCTAGACCGATTCACCATCTCCAGAGACGATTCCAAGAACACGCTGTA34620.16002055954502764No Hit
GCTGTATATTACTGTGTCAAAGATGGGTGGGCGACGTGGGGCCAGGGAAC32800.15160815577922895No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC32240.1490197238512909No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA32040.14809528387702728No Hit
GTATTTAATACTTACATCATATGATATGACTGCCACCCACTCCAGCCCCT31900.14744817589504278No Hit
GTCTAGCACGGAGTCTGCATAGTATTTAATACTTACATCATATGATATGA31610.1461077379323606No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC31370.14499840996324426No Hit
ATTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC27830.12863582241877872No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA27500.12711049646124378No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCGGGCCAGTCGCA26690.12336651456547625No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC26170.12096297063239089No Hit
CCTTCCACCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGTCG25410.11745009873018926No Hit
CAGTATAATAGTTATTCGACGTTCGGCCAAGGGACCAGGGTGGAAATCAA25230.11661810275335202No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT24730.11430700281769303No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG24080.11130257290133637No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG23930.11060924292063867No Hit
ATATTACTGTGTCAAAGATGGGTGGGCGACGTGGGGCCAGGGAACCCTGG23190.10718881501586339No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22960.10612570904546027No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22710.10497015907763077No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22480.10390705310722764No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG21680.10020929321017329No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36150.080.465291
ATCAACG44350.065.106813
TCAACGC45300.063.5824784
TATCAAC47150.061.3931662
CAACGCA51450.055.702335
AACGCAG52850.053.8192676
CGCAGAG55250.051.090428
GCAGAGT64450.043.901069
ACGCAGA66850.042.3327877
GTACATG60550.034.1274031
TACATGG60800.033.0427552
ACATGGG62950.031.5710493
TATACTG36900.030.637195
AATATAC42600.030.0874963
GTAATAT42050.029.450861
CATGGGA31450.029.3065664
GGTATCA44350.028.8976081
TTATACT39350.028.7296664
CAGAGTA63100.028.6384669
CTATTAT36400.027.8903031
Produced by FastQC (version 0.11.5)