FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2362671
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT52650.22284101341236257No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT47300.20019714975127728No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA41600.17607191183199014No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39200.1659139169186061No Hit
TCCATAGACACGTCCAAGAACCAATTCTCCCTCAAACTGACGTCTGTGAT38940.16481346746965617No Hit
GTCTCTGGCGGCTCCATCGCCAGTACTCGTTACTATTGGGGCTGGGTCCG38350.1623162937201159No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC38190.16163909405922366No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA37610.15918424528848918No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT35550.1504652996545012No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC33650.1424235536814055No Hit
GGGTATTAGTAAGTGGTTAGCCTGGTATCAGCAGAGACCAGGCAAGGCCC32320.1367943315002385No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC32010.13548225715725973No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT31340.13264648357727335No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT30290.12820236080266784No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30260.12807538586625053No Hit
CTCTGAGAGTCATGGATCTCAAGTGCAAGAAAAGGAAGCACCTGTGGTTC30110.12744051118416402No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT28970.1226154636003066No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA28170.11922946529584524No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG28110.11897551542301066No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27550.11660531660988771No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT27320.11563184209735507No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG26670.11288071847498021No Hit
GTTGGAGACAGAGTCACCATCACTTGTCGGGCGAATCAGGGTATTAGTAA26330.11144166919558415No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG26270.11118771932274954No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26080.11038354472543999No Hit
CTCCAGAGTCGAGCCACCATATCCATAGACACGTCCAAGAACCAATTCTC25090.10619337182366906No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG25040.10598174692964023No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25020.10589709697202869No Hit
GTCTTTCACAATGGAAACACCTACTACAATTCGTCCCTCCAGAGTCGAGC24780.10488129748069028No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT24520.10378084803174036No Hit
GGTCTATGCTACTTCCACTTTGCAAAGTGGAGTCCCATCAAGGTTCAGCG24410.10331527326487693No Hit
CTCTATTTCTGCGCGAGACAAGATGCGACAGCAGTGGCTGATACACGGGC24310.10289202347681925No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTAGTCCCTTGGCCGAACGTC23870.1010297244093655No Hit
GTATTAGTAAGTGGTTAGCCTGGTATCAGCAGAGACCAGGCAAGGCCCCT23840.1009027494729482No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA23830.10086042449414243No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47250.072.835461
ATCAACG49550.067.866323
TCAACGC50500.066.589634
TATCAAC53300.063.0914882
CAACGCA59700.056.327915
CGCAGAG63050.053.33628
AACGCAG64500.052.248816
GCAGAGT70100.047.859299
ACGCAGA79250.042.342547
GAATACG6050.032.1297049
GTACATG57050.030.2881181
ACATGGG57700.029.5769633
TACATGG57800.029.1520482
GGTATCA55950.028.3099651
CAGAGTA68450.027.8721689
CAGCGCT12950.027.7970439
TCTACAC19650.027.1175383
GTACACG20200.026.7356625
CATTAGC16700.025.440518
CATGGGA24150.025.344454
Produced by FastQC (version 0.11.5)