FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620457
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCGTCAGTTGTCAGGGAGCTCATGTGTAGATACA38580.6217997379351027No Hit
GTCTATTACTGTGCGTGCCGCTTAGACTATAGCAGCTCGTCGGAGATGTT26780.4316173401218779No Hit
GCTATACACTGGGTCCGCCAGGCTCCCGGCAAGGGGCTGGAGTGGGTGGC20100.32395476237676424No Hit
CTCCTGTACAGCCTCTGGATTCACCTTCAGTACCTCTGCTATACACTGGG17590.2835007099605613No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16260.2620648973256809No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG15380.24788180325147433No Hit
GTATCTACACATGAGCTCCCTGACAACTGACGACACGGCTGTCTATTACT14810.23869502640795412No Hit
GTGTATAGCAGAGGTACTGAAGGTGAATCCAGAGGCTGTACAGGAGAGTC12830.20678306474098931No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12690.2045266634110019No Hit
CTGCTATACACTGGGTCCGCCAGGCTCCCGGCAAGGGGCTGGAGTGGGTG11700.18857068257751947No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11700.18857068257751947No Hit
GTGTAGATACAGCGTGTTCCTGGAATTGTCTCTGGAGATGGTGAATCGGC11430.18421905144111517No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10940.17632164678615922No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10850.17487110307402448No Hit
CAGTAATAGACAGCCGTGTCGTCAGTTGTCAGGGAGCTCATGTGTAGATA10730.1729370447911781No Hit
GATAAATACTACGCAGACTCCGTTGAGGGCCGATTCACCATCTCCAGAGA10410.16777955603692116No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10360.16697369841906853No Hit
GCTATAGTCTAAGCGGCACGCACAGTAATAGACAGCCGTGTCGTCAGTTG10260.16536198318336323No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10170.16391143947122847No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9800.15794809309911886No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9570.15424114805699668No Hit
GTATTTATCACTTCCATCATAGGAGATAACTGCCACCCACTCCAGCCCCT9400.1515012321562977No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9130.1471496010198934No Hit
GTATAGCAGAGGTACTGAAGGTGAATCCAGAGGCTGTACAGGAGAGTCTC8850.14263679835991858No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8790.14166976921849542No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC8770.14134742617135435No Hit
TGTGTAGATACAGCGTGTTCCTGGAATTGTCTCTGGAGATGGTGAATCGG8740.14086391160064274No Hit
CTATTACTGTGCGTGCCGCTTAGACTATAGCAGCTCGTCGGAGATGTTTC8590.1384463387470848No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8420.13570642284638582No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8260.13312767846925735No Hit
ATCTACACATGAGCTCCCTGACAACTGACGACACGGCTGTCTATTACTGT8000.12893721885642356No Hit
GGGCTGGAGTGGGTGGCAGTTATCTCCTATGATGGAAGTGATAAATACTA7760.12506910229073087No Hit
GACTATAGCAGCTCGTCGGAGATGTTTCAGTCCTGGGGCCAGGGAACCCT7690.12394090162573716No Hit
GTACAGGAGAGTCTCAGGGACCTCCCAGGCTGGACCACGCCTCCCCCAGA7670.1236185585785961No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG7610.12265152943717293No Hit
ATACTACGCAGACTCCGTTGAGGGCCGATTCACCATCTCCAGAGACAATT7570.12200684334289082No Hit
CTCCAGCCCCTTGCCGGGAGCCTGGCGGACCCAGTGTATAGCAGAGGTAC7550.12168450029574976No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT7260.1170105261122044No Hit
AGTCTAAGCGGCACGCACAGTAATAGACAGCCGTGTCGTCAGTTGTCAGG7180.11572115392364016No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT7140.11507646782935804No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACTCAGGCTG6990.11265889497580009No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC6720.10830726383939579No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC6690.10782374926868422No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT6510.10492266184441469No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG6500.10476149032084416No Hit
CCCTGAGACTCTCCTGTACAGCCTCTGGATTCACCTTCAGTACCTCTGCT6490.10460031879727363No Hit
GTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC6420.10347211813227991No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC6410.10331094660870938No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG6290.10137688832586303No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCC6290.10137688832586303No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC6230.10040985918443986No Hit
TGTAGATACAGCGTGTTCCTGGAATTGTCTCTGGAGATGGTGAATCGGCC6220.10024868766086933No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAACG450.00989497548.011722
GAATCGT803.8886705E-545.00016
CAGCCGT17000.037.270679
TAGACAG18400.036.4001925
CAGCGCT3600.036.000089
AATAGAC18200.035.217393
GTAATAG18500.035.041231
ATAGACA18600.034.4600264
TAATAGA18450.034.349852
GGCAGCG4100.033.365937
GTATCAA14200.032.971111
GCAGCGC4200.032.57158
TCAACGC13650.032.183684
CCGGACT900.003747711432.0078124
AGACAGC21100.031.3934356
GTCTAGT1153.283813E-431.3170411
TATCAAC14150.031.046452
ATCAACG14400.030.5074463
CAACGCA14650.029.9868435
AACGCAG14750.029.7763376
Produced by FastQC (version 0.11.5)