FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2365827
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTTCCTCCGCCGAATACCAC45330.19160318992048023No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38050.16083170916554762No Hit
GATGTACACTGGTACCAACAATTTCCAGGAACAGCCCCCAAACTCCTCAT36470.15415328339730675No Hit
GTAATAAACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGAAAAA32970.13935930226512758No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGGCTGGAGCCCAG31570.13344170981225592No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27890.11788689536470756No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA25980.10981360851828981No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA25660.10846101595763341No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25470.1076579141247437No Hit
TCCTATGACAGCAGCCTGACTGGTGTGGTATTCGGCGGAGGAACCAAGCT24520.10364240496029506No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC24080.10178259018939254No Hit
GGTTATGATGTACACTGGTACCAACAATTTCCAGGAACAGCCCCCAAACT23880.10093721983898231No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)