FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2365827
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTTCCTCCGCCGAATACCAC37490.15846467218439894No Hit
GATGTACACTGGTACCAACAATTTCCAGGAACAGCCCCCAAACTCCTCAT33890.1432480058770147No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33100.13990879299289424No Hit
GTAATAAACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGAAAAA29890.12634059886880994No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCC27280.11530851579595634No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26330.11129300663150772No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGGCTGGAGCCCAG24830.10495272900343093No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35350.069.049771
TCAACGC41150.057.571234
ATCAACG42050.056.681523
CAACGCA44500.053.0754055
GGTATCA17950.052.147181
TATCAAC45750.052.097442
AACGCAG47450.049.775676
CGCAGAG54400.043.548838
GCAGAGT65500.036.0520069
GTACATG64250.034.516721
TACATGG64950.033.481682
ACATGGG66650.032.195533
ACGCAGA82050.028.8733237
CAGAGTA63650.025.1102719
CATGGGA33000.024.6572134
CTAGTAC3801.4551915E-1124.6342433
CATGGGG46950.023.619174
TATTGAG22050.023.186185
TTATTGA26050.021.5608754
TAAACAG33500.021.2799135
Produced by FastQC (version 0.11.5)