FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767347
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCGTCAGATCTCAGGCTCCTCAGCTCCAGATACA22790.2969973167289375No Hit
GTGTATCTGGAGCTGAGGAGCCTGAGATCTGACGACACGGCCGTGTATTA21010.27380051006910827No Hit
GTGTATTACTGTGCGAGAGATAATGTGGGGGATATTTACTATTACTACGG16630.21672072738930367No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC16530.21541753600392002No Hit
GTCTGGAGCTGTGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA15510.2021249838730066No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13490.17580051788825654No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13420.17488828391848799No Hit
CCAGATACACTGTGCGCGTGGATGTGTCTGTGGTCATGGTGGCTCTGCCC12990.16928456096133823No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC12680.16524466766664886No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11800.1537765834752726No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA11580.15090956242742853No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11450.14921541362642976No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGATGGTTACCA11280.14699998827127753No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10990.1432207332536649No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCAGGATCAC10370.13514094666428617No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA9610.1252366921353703No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGG9320.12145743711775767No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9280.1209361605636042No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9260.12067552228652748No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9140.11911169262406708No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCG9120.11885105434699035No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9010.11741754382306831No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8870.11559307588353118No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCC8800.11468084191376261No Hit
CTCCCAGGTTCAGCTGGTGCAGTCTGGAGCTGTGGTGAAGAAGCCTGGGG8730.11376860794399404No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT8710.11350796966691731No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8370.10907711895661286No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8360.10894679981807448No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA8320.108425523263921No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC7980.10399467255361655No Hit
GTAATAGTCAGCCTCATCTGCCGCCTGAGCCCCAGTGATGGTCAAGGAAG7770.10125797064431084No Hit
GTATCAACGCAGAGTACATGGGACCCAAAAACCACACCCCTCCTTGGGAG7770.10125797064431084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)