FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901860
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCGTCACCTCTCAGGCTGTTCATTTGCAGATACA37740.4184684984365645No Hit
GTTTATTACTGTGCGATGAAGGGAGAATATCAGATGCAAAATAGTGAATA31370.34783669305657194No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC27860.3089171268267802No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCTCTCAGG25500.2827489854301111No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT24700.2738784290244605No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGTTGGTGGAGTCTGGGGGAGGCG23370.2591311290000665No Hit
CTCCTGCACTGGGAGCAGTTCCAACATCGGGGCACATTATGATGTACACT21730.2409464883684829No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT19650.2178830417137915No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC18580.20601867252123388No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCT17850.19792428980107776No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG17780.19714811611558336No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA17630.19548488678952386No Hit
TCCTATGACAGCAGCCTGAGAGGTGTGGTATTCGGCGGAGGGACCAAGCT17310.19193666422726366No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA17200.1907169627214867No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT16230.17996141307963542No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC16130.1788525935289291No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT16050.17796553788836403No Hit
TTCCAGTACTGGGGCCAGGGTACCCTGGTCACCGTCTCCTCAGCATCCCC15900.17630230856230455No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15640.17341937773046814No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15490.17175614840440867No Hit
CCTCTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCCTCATCCTC15330.16998203712327856No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA14970.16599028674073582No Hit
ATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTC14930.16554675892045329No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14760.16366176568425253No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCTCTCAGGC14620.1621094183132637No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14620.1621094183132637No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA14560.1614441265828399No Hit
GTTCCAACATCGGGGCACATTATGATGTACACTGGTACCAGCAGCTTCCA14420.15989177921185105No Hit
CATTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACT14240.15789590402057968No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13630.15113210476127115No Hit
GTACTGGAAATATTCACTATTTTGCATCTGATATTCTCCCTTCATCGCAC13590.1506885769409886No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA13570.15046681303084736No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13500.14969063934535293No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13440.14902534761492914No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13420.14880358370478788No Hit
ATATTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATT13390.148470937839576No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA13150.14580977091788083No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG13140.1456988889628102No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13070.14492271527731576No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13000.14414654159182136No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC12950.14359213181646818No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12860.1425941942208325No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT12790.14181802053533807No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC12770.14159625662519681No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12670.1404874370744905No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12610.1398221453440667No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA12590.13960038143392545No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12580.1394894994788548No Hit
CTCCTGTGTAGCCTCTGGATTCACCTTCAGTACCGAGGCTGTGCACTGGG12230.13560863105138268No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT12220.13549774909631207No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT11990.13294746412968753No Hit
GGGTACCCTGGCCCCAGTACTGGAAATATTCACTATTTTGCATCTGATAT11950.13250393630940502No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG11660.12928835961235668No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11480.12729248442108532No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC11480.12729248442108532No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG11430.12673807464573217No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11430.12673807464573217No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA11100.1230789701284013No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10990.12185926862262435No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT10950.12141574080234183No Hit
GGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAAC10910.12097221298205929No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC10890.12075044907191804No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGAGGTGTGGTATTCGG10700.11864369192557604No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG10540.11686958064444591No Hit
TCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC10330.11454105958796267No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA10100.11199077462133813No Hit
GTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGTT9930.11010578138513738No Hit
CTGCTACCCAGAGCAATATCTGTGATGAAGACAGTGCTACAGAGACCTGC9880.10955137160978423No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG9830.10899696183443107No Hit
GTGTAGAGAACATATATGCATAAACATAGGTCAATTATATGTCTCCATTA9760.10822078814893665No Hit
GTACCGAGGCTGTGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAG9750.108109906193866No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9590.1063357949127359No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC9590.1063357949127359No Hit
ATACCACACCTCTCAGGCTGCTGTCATAGGACTGGCAGTAATAATCAGCC9580.10622491295766527No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9510.10544873927217084No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG9450.10478344754174705No Hit
TTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCC9320.10334198212582885No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9180.10178963475483999No Hit
GCCTGAGAGGTGACGACACGGCTGTTTATTACTGTGCGATGAAGGGAGAA9130.10123522497948684No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCA9120.10112434302441621No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG9120.10112434302441621No Hit
GGGCTGGAGTGGGTGGCAGTTATATCATATGATGGAAGTGAGAAATATTA9090.10079169715920432No Hit
GTATCTGCAAATGAACAGCCTGAGAGGTGACGACACGGCTGTTTATTACT9080.10068081520413369No Hit
GTCCAGTGTCAGGTGCAGTTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC9020.10001552347370989No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5250.090.7268141
GTATCAA20450.071.63971
TCAACGC22100.064.5649264
ATCAACG22550.063.2764973
CAACGCA23000.062.035035
TATCAAC22900.061.994692
AACGCAG23700.060.4397286
ACGCAGA26850.053.3431177
GTGCTAG5350.052.609221
CGCAGAG27450.052.177158
GTGTAGA4800.051.1197431
GAATACG5450.048.8624049
GCAGAGT29600.048.3872539
GTACATG24800.039.8675841
AGAATAC7050.039.814858
GCTAGAG6900.039.6879123
TACATGG25050.039.4127352
CTAGAGA7150.039.3081254
GTGCGCA750.001537447238.38569
TGTAGAG5700.037.928892
Produced by FastQC (version 0.11.5)