FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences943498
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATTACTGTGCGAGAGATCGCGGTCGGGATGGTTCGGGGAGTTATCC54390.5764718102211133No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA48550.5145744877042665No Hit
ATATTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACAACC28980.30715486413325727No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA23410.24811923289715507No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT23020.24398567882496836No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21910.23222094800412932No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC21370.22649756544264005No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC20920.22172807997473234No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20760.22003226291947625No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC19780.20964538345603279No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA18540.19650280127779815No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCCTCC17350.18389016192933105No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG16840.17848474506570233No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16250.17223141967444552No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG15200.1611026202493275No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14550.15421336346234968No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14340.15198760357732607No Hit
ATATAACATGAATTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGG14200.15050376365397702No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT14020.14859596946681392No Hit
CTCCAGAGACAACCCCAGGAACTCACTGTATCTGCAAATGAACAGCCTGA13920.14753608380727887No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13810.1463702095817903No Hit
GCGTAATATATGTAACTATTACTACTACTAATGGATGAGACCCACTCCAG13420.14223665550960363No Hit
CTTCTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT13370.1417067126798361No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT13300.14096479271816156No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC13070.13852705570123097No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12830.13598333011834685No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC12570.1332276274035557No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12430.13174378748020663No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12410.13153181034829964No Hit
CCGTAGAAGTGGTAGTCAAAGGGGGGATAACTCCCCGAACCATCCCGACC12000.1271862791442059No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC11960.12676232488039188No Hit
GTTACATATATTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGA11710.12411261073155429No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCTTAGCCCTGGATTCCAAGGCCTA11620.12315871363797273No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA11560.1225227822422517No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA11350.1202970223572281No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG11200.11870719386792553No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT10960.11616346828504141No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG10940.11595149115313441No Hit
CCCTGAGACTCTCCTGTGCGGCCTCTGGATTCACCTTCAGTCGATATAAC10690.11330177700429678No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG10590.11224189134476173No Hit
GTGTGAGGTGCTTCTGGTGGAGTCTGGGGGAGGCCTGGTCAAGCCTGGGG10410.11033409715759863No Hit
GTCTGGGGGAGGCCTGGTCAAGCCTGGGGGGTCCCTGAGACTCTCCTGTG10390.11012212002569162No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG10320.1093802000640171No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGACT10290.10906223436615659No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10190.10800234870662154No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC10140.10747240587685401No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT10010.10609455451945843No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT9960.10556461168969092No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT9930.1052466459918304No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9860.10450472603015587No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9700.10280890897489978No Hit
CTCCTGTGCGGCCTCTGGATTCACCTTCAGTCGATATAACATGAATTGGG9690.10270292040894628No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC9670.10249094327703927No Hit
CTATTACTGTGCGAGAGATCGCGGTCGGGATGGTTCGGGGAGTTATCCCC9650.10227896614513227No Hit
TAGTAATAGTTACATATATTACGCAGACTCAGTGAAGGGCCGATTCACCA9600.10174902331536474No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9590.10164303474941123No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC9540.10111309191964371No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17950.077.5748141
ATCAACG20250.068.6693
TCAACGC20300.068.499864
CAACGCA20550.067.666535
TATCAAC21050.066.401532
AACGCAG21700.064.344266
GGTATCA5100.059.416491
CGCAGAG25950.053.8061878
GCAGAGT28450.048.825079
CAGCGCT6300.044.554539
GAATACG2750.039.257849
GCAGCGC7400.036.9589588
GGCAGCG7900.036.441887
GTACATG24450.033.6398351
TACATGG24600.033.3885962
ACATGGG25550.032.147143
CAGCCGT21100.032.063679
GTGCTAG4000.030.6631531
TCTACAC6150.030.4597723
ATAGACA22950.030.4521144
Produced by FastQC (version 0.11.5)