FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965392
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC27790.2878623398577987No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATAAGAACCA21350.22115368679251537No Hit
GTAATATGTGGCTGAGTCCACAGGGTCCATGTTGGCCATTGTAAGGACTA20530.21265972786184265No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17500.18127351376435685No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA17010.17619785537895488No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT15230.15775974940749457No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA15130.15672390075741255No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT14750.15278767588710077No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14490.1500944693968875No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT13990.14491522614647728No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCGCCTATGAGCTGACACAGCCA13860.14356862290137062No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13170.13642126721580455No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12880.13341730613056663No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12720.13175994829043539No Hit
CTCTCTGCACAGGCTCTGAGGCCGCCTATGAGCTGACACAGCCACCCTCG12710.1316563634254272No Hit
CAGTAATATGTGGCTGAGTCCACAGGGTCCATGTTGGCCATTGTAAGGAC12330.12772013855511544No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAG12320.12761655369010724No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCAC12300.1274093839600908No Hit
ACATATTACTGTGCACACATTGATTCGGGTGGCTACGACCCGTGGTTCGA12200.12637353531000878No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12180.1261663655799924No Hit
GGCTCTGAGGCCGCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTC12110.12544127152493495No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12020.12450900773986111No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11930.12357674395478728No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC11880.12305881962974625No Hit
ATATTACTGTGCACACATTGATTCGGGTGGCTACGACCCGTGGTTCGACC11640.12057278286954937No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA11620.12036561313953295No Hit
GTCTTTAAATATCACCAACACAGGGGCCTGGCCTGGCTTCTGCTGGTACC11530.11943334935445912No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11420.11829391583936888No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11170.11570429421416378No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11170.11570429421416378No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11160.11560070934915558No Hit
GTGTTGGTGATATTTAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCG10930.1132182574539669No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA10870.11259674826391765No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG10730.1111465601538028No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA10620.11000712663871257No Hit
GTGATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA10610.10990354177370437No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT10460.1083497687985813No Hit
TTTAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTC10310.10679599582345825No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG10250.10617448663340903No Hit
CATATTACTGTGCACACATTGATTCGGGTGGCTACGACCCGTGGTTCGAC9950.10306694068316291No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)