FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences965392
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC23480.2432172630392628No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATAAGAACCA18760.19432520675539056No Hit
GTAATATGTGGCTGAGTCCACAGGGTCCATGTTGGCCATTGTAAGGACTA16870.17474766726884003No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA14430.14947296020683826No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14030.14532956560651009No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA13090.13559258829573892No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT12780.1323814574804846No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12640.13093126937036975No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT12280.1272022142300744No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCGCCTATGAGCTGACACAGCCA12090.12523410179491853No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT11740.1216086315196314No Hit
GTCTTTAAATATCACCAACACAGGGGCCTGGCCTGGCTTCTGCTGGTACC11540.11953693421946732No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11280.11684372772925403No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11230.11632580340421302No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCAC11090.11487561529409814No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC10860.11249316339890945No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10560.10938561744866335No Hit
ACATATTACTGTGCACACATTGATTCGGGTGGCTACGACCCGTGGTTCGA10510.10886769312362231No Hit
CTCTCTGCACAGGCTCTGAGGCCGCCTATGAGCTGACACAGCCACCCTCG10450.1082461839335731No Hit
GTGTTGGTGATATTTAAAGACAGTGAGAGGCCCTCAGGGATCCCTGAGCG10210.10576014717337621No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10170.1053458077133434No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA10130.10493146825331058No Hit
CAGTAATATGTGGCTGAGTCCACAGGGTCCATGTTGGCCATTGTAAGGAC9970.1032741104131793No Hit
GGCTCTGAGGCCGCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTC9850.10203109203308088No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGCCAAAG9710.100580903922966No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13600.052.4845771
TCAACGC13450.048.1654054
ATCAACG13600.048.163443
TATCAAC15000.044.1480522
CAACGCA14650.043.7287835
AACGCAG15700.040.8042456
CGCAGAG16800.038.5609978
TACATGG24850.032.1522672
GCAGAGT20750.031.9142559
ACATGGG24950.031.1579023
GTACATG26150.030.8803161
ACGCAGA21200.030.2182397
GGTATCA8200.029.895131
TACGGGA1050.00798358527.4211522
GTCCTAT2456.79247E-826.4857251
GTATTAC3006.2573235E-1026.4366741
CTAAGTC20700.026.4276313
CATGGGG17550.026.2493084
TATTGAG10850.025.8731845
CCTAAGT21850.025.6955662
Produced by FastQC (version 0.11.5)