FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846670
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATAGTGGGGACACCTACTACAACCCGTCCCTCAAGAGTCGAGTCA98380.5327427206810096No Hit
GTAATAAACAGCCGTATCAGCGACGGTCACATAAGGCAACCTCAGGGAGA94880.5137896863002052No Hit
GTTTATTACTGTGCGAGACAACGGGGGAACTGGTTCGACCCCTGGGGCCA94470.511569473701311No Hit
GTCTCTGGTGTCTCCATCAGCAGTAGTAATTACTACTGGGGCTGGATCCG61710.33416907189698214No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT60050.32517991844780064No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT52600.2848370309800885No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA51810.2805590603627069No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT51800.2805049088359046No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC51370.2781763931834058No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC47300.2561367217748704No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45380.24573962862882917No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA43650.2363714144920316No Hit
CTATAAGAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG42760.23155192860662707No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC41630.22543280607796737No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG40820.22104653240698122No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG40540.21953028965651686No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGACGACACGTCC39760.2153064705659376No Hit
CCTTAGTAGTTACGTTTTCACCTTCGGCCAAGGGACACGACTGGAGATAA38310.20745449917960435No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT37290.20193104344576998No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32780.17750870485793346No Hit
ACTTATTACTGTCAACACCTTAGTAGTTACGTTTTCACCTTCGGCCAAGG31860.17252676439212203No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG31500.1705773094272393No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT30650.16597442964904396No Hit
CACCTGCACTGTCTCTGGTGTCTCCATCAGCAGTAGTAATTACTACTGGG29330.1588264281111406No Hit
CTACTAAGGTGTTGACAGTAATAAGTTGCATAATCTTCAGGCTGCAGGCT28380.15368203306492226No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA28380.15368203306492226No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC27970.15146182046602805No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA27840.15075785061759817No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGG27690.1499455777155637No Hit
TTATTACTGTGCGAGACAACGGGGGAACTGGTTCGACCCCTGGGGCCAGG27660.1497831231351568No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG27620.1495665170279476No Hit
TATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGAT27390.14832103191149473No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT27290.14777951664347178No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27010.14626327389300742No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA26480.14339324297248562No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG26380.1428517277044626No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG26320.14252681854364885No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26220.14198530327562586No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC26010.1408481212127776No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG25870.14008999983754544No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25650.13889866624789488No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC25530.1382488479262673No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC25450.1378156357118489No Hit
GTAATAAGTTGCATAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGA25370.13738242349743052No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG25330.13716581739022132No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24990.1353246654789432No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT24940.13505390784493168No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24920.13494560479132708No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC24710.13380842272847882No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT24340.1318048162367938No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC24330.1317506647099915No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCTTCACTT24240.1312633009687708No Hit
GATCTATGATGCATCCACTTTGCAAAGTGGAGTCCCATCAAGATTCAGCG23970.12980120974510875No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC23860.1292055429502835No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT23700.1283391185214467No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGACGACAC23600.12779760325342374No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT23350.12644381508336627No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG23310.12622720897615708No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG23280.12606475439575018No Hit
GGATTGGGAGTATCTCTTATAGTGGGGACACCTACTACAACCCGTCCCTC23220.1257398452349364No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT22980.12444020859168124No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT22370.12113696545674106No Hit
TCCCAGATGGGTCCTGTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGAC21790.11799617690220776No Hit
CAGTAATAAACAGCCGTATCAGCGACGGTCACATAAGGCAACCTCAGGGA21400.11588426735691813No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC21350.11561350972290664No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT21200.11480123682087216No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA21070.1140972669724423No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC20970.1135557517044193No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC20290.10987344788186303No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCTTCACTTGCCGGGCCAGTCAGG20260.10971099330145614No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20250.10965684177465383No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA20140.10906117497982855No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG20060.10862796276541017No Hit
CACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA20040.10851965971180558No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19880.1076532352829688No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA19860.1075449322293642No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC19840.10743662917575962No Hit
GTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCA19460.10537887115727228No Hit
GTGGGAGACAGAGTCACCTTCACTTGCCGGGCCAGTCAGGGCATTAGTAC19220.10407923451401713No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA19090.10337526466558725No Hit
ATCTCTTATAGTGGGGACACCTACTACAACCCGTCCCTCAAGAGTCGAGT19060.10321281008518035No Hit
GTCAACACCTTAGTAGTTACGTTTTCACCTTCGGCCAAGGGACACGACTG18890.10229223412954128No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA18710.10131750664709992No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC18640.10093844595948381No Hit
CTTATAGTGGGGACACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACC18620.10083014290587923No Hit
CTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACAC18530.10034277916465854No Hit
TGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCT18510.10023447611105395No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)