FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846670
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTATCAGCGACGGTCACATAAGGCAACCTCAGGGAGA87570.47420492020772526No Hit
GTTTATTACTGTGCGAGACAACGGGGGAACTGGTTCGACCCCTGGGGCCA83760.45357318849604966No Hit
CTCTTATAGTGGGGACACCTACTACAACCCGTCCCTCAAGAGTCGAGTCA83540.4523818549063991No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT56050.30351930772688135No Hit
GTCTCTGGTGTCTCCATCAGCAGTAGTAATTACTACTGGGGCTGGATCCG55480.30043267069915036No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT53780.29122691114275967No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT47010.25456632749760383No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA43620.2362089599116247No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC42810.23182268624063856No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC42660.2310104133386041No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA41160.22288768431825934No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40660.2201801079781444No Hit
CTATAAGAGATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCG39450.21362777323506635No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC38480.20837507513524345No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG37180.2013353766509447No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGACGACACGTCC34100.1846567063958368No Hit
CCTTAGTAGTTACGTTTTCACCTTCGGCCAAGGGACACGACTGGAGATAA33500.18140761478769896No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT31690.17160618843648298No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG31220.16906106667677495No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT30950.16759897545311292No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30230.16370006552334743No Hit
ACTTATTACTGTCAACACCTTAGTAGTTACGTTTTCACCTTCGGCCAAGG29760.16115494376363942No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG29590.16023436780800035No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA29340.1588805796379429No Hit
CTACTAAGGTGTTGACAGTAATAAGTTGCATAATCTTCAGGCTGCAGGCT28660.15519827581538662No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT27050.1464798800002166No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT26920.14577591015178673No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25960.1405773635787661No Hit
CACCTGCACTGTCTCTGGTGTCTCCATCAGCAGTAGTAATTACTACTGGG25770.13954848456952243No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG25720.13927772693551094No Hit
TATTTAGCCTGGTATCAGCAAAAACCAGGGAAAGCCCCTAAGCTCCTGAT25230.13662430212219834No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25220.13657015059539604No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGG24830.1344582410501064No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC24500.13267124066563057No Hit
TTATTACTGTGCGAGACAACGGGGGAACTGGTTCGACCCCTGGGGCCAGG24360.1319131192903984No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA24150.13077593722755013No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24070.13034272501313174No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT23790.12882648226266738No Hit
GTAATAAGTTGCATAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGA23580.12768930019981914No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC23120.12519832996691344No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC23120.12519832996691344No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA22970.12438605706487893No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT22850.12373623874325138No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22800.12346548110923987No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22740.12314057194842609No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG22460.12162432919796175No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG21650.11723805552697557No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG21420.11599257041052273No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCTTCACTT21400.11588426735691813No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC21360.11566766124970894No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGACGACAC21010.1137723578116285No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20990.1136640547580239No Hit
GATCTATGATGCATCCACTTTGCAAAGTGGAGTCCCATCAAGATTCAGCG20740.11231026658796645No Hit
GGATTGGGAGTATCTCTTATAGTGGGGACACCTACTACAACCCGTCCCTC20550.11128138757872277No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC20310.10998175093546762No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT20130.10900702345302626No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC20030.10846550818500328No Hit
TCCCAGATGGGTCCTGTCCCAGCTGCAGCTGCAGGAGTCGGGCCCAGGAC19960.10808644749738719No Hit
CAGTAATAAACAGCCGTATCAGCGACGGTCACATAAGGCAACCTCAGGGA19740.10689511390773662No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA19010.10294205245116887No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC18900.10234638565634359No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG18890.10229223412954128No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT18790.1017507188615183No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)