FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2339213
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC96490.4124891576782448No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGTTCTG72020.30788132589892414No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC69970.29911769471185395No Hit
GACCTAGGACGAGCAGCTTGGTCCCTCCGCCGAATACTACATGGTCATTA68050.29090980599030525No Hit
GCCTTGGGCTGACCTAGGACGAGCAGCTTGGTCCCTCCGCCGAATACTAC64790.2769734949318424No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT57320.2450396778745672No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC49660.21229362182922204No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT49000.2094721600811897No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT44670.1909616610372805No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA44440.18997842436751164No Hit
CATATGTGGTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACG44360.1896364290041138No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG42960.18365151014465123No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA42570.18198428274808665No Hit
ATATATTACTGTGCGAAGTCCGGTGATAGCAGTCCAATCGACTACGTAAT41100.17570011794565096No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT39480.16877471183684428No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA39240.1677487257466507No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA38820.16595325008881193No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA38460.16441427095352154No Hit
GCCTTCAGGGATCCCTGAGCGAATCTCTGGCTCCAACTCTGGGAACATGG38290.1636875308063011No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT37560.1605668231152956No Hit
GTAATATATGGCGGTGTCCGAGGCCCTCAGGCTGCTCCACTGCAAGTAGG37030.15830110383278478No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG36850.1575316142651396No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC36330.1553086444030535No Hit
ATCCCACACCTGACAATAATAGTCGGCCTCATCCCCGCCTTCGACCCTGC36330.1553086444030535No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA36310.15522314556220404No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34660.14816949119212316No Hit
CCTCTCTCACTGCACAGGTTCTGTGACCTCATATGTGGTGACTCAGCCAC34510.14752824988575217No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT34510.14752824988575217No Hit
GGTTCTGTGACCTCATATGTGGTGACTCAGCCACCCTCAGTGTCAGTGGC34290.14658776263640805No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCCGTGTTGGTCATTTATTATGAT33500.14321055842285418No Hit
CTATTATTGTCAGGTGTGGGATAATGACCATGTAGTATTCGGCGGAGGGA32170.13752488550636474No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG32110.13726838898381635No Hit
GTAGTATTCGGCGGAGGGACCAAGCTGCTCGTCCTAGGTCAGCCCAAGGC30990.1324804538962463No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA30260.12935974620524082No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA28960.12380232155002559No Hit
GATAATGACCGGCCTTCAGGGATCCCTGAGCGAATCTCTGGCTCCAACTC28400.12140835400624055No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA28370.12128010574496637No Hit
CAATAATAGTCGGCCTCATCCCCGCCTTCGACCCTGCTGATGGTCAGGGT28340.12115185748369217No Hit
GTCATTATCCCACACCTGACAATAATAGTCGGCCTCATCCCCGCCTTCGA27700.11841589457650928No Hit
CTACATGGTCATTATCCCACACCTGACAATAATAGTCGGCCTCATCCCCG27190.11623567413484792No Hit
GTGTACACCTGTGGCTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT27170.11615017529399846No Hit
GTGTGGGATAATGACCATGTAGTATTCGGCGGAGGGACCAAGCTGCTCGT27090.1158081799306006No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG27090.1158081799306006No Hit
GGATTACCTGTGGGGGAAACAATATTGGAAGTAAAATTGTGCACTGGTAC26970.1152951868855038No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC26940.11516693862422961No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC26350.11264472281917039No Hit
CCCTCAGTGTCAGTGGCCCCAGGAAAGACGGCCAGGATTACCTGTGGGGG26140.111746984990251No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG26100.11157598730855206No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG25860.11055000121835848No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG25700.10986601049156276No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT25040.10704454874353041No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC24970.10674530280055727No Hit
ACCATGTAGTATTCGGCGGAGGGACCAAGCTGCTCGTCCTAGGTCAGCCC24650.10537732134696584No Hit
ATTATTGTCAGGTGTGGGATAATGACCATGTAGTATTCGGCGGAGGGACC24300.1038810916321002No Hit
CCTCATATGTGGTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAG24270.103752843370826No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA24200.10345359742785287No Hit
GTATCAACGCAGAGTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGT23860.10200011713341196No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG23710.10135887582704098No Hit
ATACTACATGGTCATTATCCCACACCTGACAATAATAGTCGGCCTCATCC23420.10011914263472373No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17050.071.789151
GTATCAA43750.071.588851
ATCAACG50050.061.2921373
TCAACGC50650.060.708244
TATCAAC53250.057.7440832
CAACGCA56400.054.390175
AACGCAG58500.052.4388286
CGCAGAG63300.048.6889088
ACGCAGA69850.043.9170467
GCAGAGT74950.041.208129
GAATACG5450.036.9878129
GTACATG65500.032.097841
TATTGAG39050.031.9017895
CATGGGA33750.031.791554
ACATGGG65250.030.7909373
TATGGCG20900.029.6307147
TTATTGA41850.029.4238764
CTGTGCG25300.028.4561799
CAGAGTA69800.027.3330149
GTAATAT25950.027.190861
Produced by FastQC (version 0.11.5)