FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911458
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGGCAGTC51940.5698562084045562No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT38830.4260207272304374No Hit
ATAATACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA30090.3301304064476915No Hit
ACAGTACCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA29160.319926974144722No Hit
GATCTATACTACATCCAGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGTG26850.29458296487605573No Hit
ATACTACATCCAGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGT25460.27933267358452063No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25290.27746753004526814No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22740.2494903769564807No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGC21770.23884808735015767No Hit
CAATAATACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGA19710.21624693622745098No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT19620.21525950729490553No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA19270.21141950589056213No Hit
GAGTTACAGTACCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCA18280.20055778763256232No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18200.19968007302585528No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18150.19913150139666339No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17630.19342635645306752No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGGAAG17520.19221949886884532No Hit
GTATTTAAATTGGTATCACCAGAAACCAGGGAAAGCCCCTAAGCTCCTGA17380.19068349830710796No Hit
GTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGGTGATACCAATT17360.1904640696554312No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT16670.18289378117258284No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16330.17916349409407784No Hit
GTGTATTATTGTGCGAGACTAGGGACCTATGGTGGCTACGATCCCCAGGG16030.17587206431892638No Hit
GTATTATTGTGCGAGACTAGGGACCTATGGTGGCTACGATCCCCAGGGGG15850.1738972064538355No Hit
CTCTAAACTGGATGTAGTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT15770.17301949184712845No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT14830.16270634521832053No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAG14530.15941491544316908No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14340.15733034325223982No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT14160.15535548538714894No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG14030.15392919915124997No Hit
ATACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGAGAATT13560.14877262583684603No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT13440.14745605392678543No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13180.1446034814549875No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12610.13834976488219972No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12500.1371429072979775No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12250.13440004915201798No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT12220.13407090617450285No Hit
AGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGAC11970.13132804802854328No Hit
TTACAGTACCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC11750.12891433286009887No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG11710.12847547555674535No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA11680.1281463325792302No Hit
CCTTCCCAGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG11540.12661033201749283No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA11470.12584233173662418No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG11410.1251840457815939No Hit
ATGTAGTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGGTGATAC10870.11925947218632126No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG10830.11882061488296773No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10820.11871090055712934No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10750.11794290027626066No Hit
GTTTAGAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACA10670.11706518566955361No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG10610.11640689971452332No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCGTACACTTTTGGCCAGGG10590.11618747106284656No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG10540.11563889943365464No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA10240.11234746965850317No Hit
ACCATAGACAGCGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCAACAGTC10150.11136004072595776No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA9680.1062034674115538No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGG9640.10576461010820028No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAAC9640.10576461010820028No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA9470.10389946656894779No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGA9350.10258289465888719No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9210.10104689409714984No Hit
GCTGTGTATTATTGTGCGAGACTAGGGACCTATGGTGGCTACGATCCCCA9170.10060803679379632No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC9160.10049832246795792No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9120.1000594651646044No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAC300.00199058871.990653
GTATCAA20500.067.5996861
TACCGTT502.0814297E-457.5925145
ATCAACG24800.056.024983
TATCAAC25550.054.1491472
CAACGCA25850.054.0277985
TCAACGC26000.053.4392134
AACGCAG27000.051.7266126
CGCAGAG29750.046.4611938
GCAGAGT32550.042.464539
ACGCAGA37050.037.5011987
GAATACG1102.5389087E-432.7230229
GTACATG28700.032.69331
GTCTATG23400.031.7701021
TACATGG29050.031.5020542
ATAGGAC1601.9830331E-631.4959093
TCTATGG23550.031.2097682
CAGCGCT6250.031.099969
ACATGGG29000.031.030453
GGTGGGT25050.030.7504967
Produced by FastQC (version 0.11.5)