FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911458
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATGGTGGGTCCTTCAGTGGTTACTACTGGAGCTGGATCCGGCAGTC46120.5060024707666179No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT33640.36907899212031714No Hit
ATAATACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA24620.2701166702140965No Hit
ACAGTACCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA23150.2539886643158544No Hit
GATCTATACTACATCCAGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGTG23090.2533303783608241No Hit
ATACTACATCCAGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGT20800.2282057977438346No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20270.22239093847440036No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19230.2109806485872086No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT17250.18925721207120896No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA17210.18881835476785544No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGC16890.18530749634102722No Hit
GTATTTAAATTGGTATCACCAGAAACCAGGGAAAGCCCCTAAGCTCCTGA16780.18410063875680505No Hit
GTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGGTGATACCAATT16450.18048006600413843No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15590.17104463398203756No Hit
GTATTATTGTGCGAGACTAGGGACCTATGGTGGCTACGATCCCCAGGGGG15380.16874063313943155No Hit
CAATAATACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGA15150.16621720364514875No Hit
GAGTTACAGTACCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCA15100.16566863201595686No Hit
CTCTAAACTGGATGTAGTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT14980.16435206010589626No Hit
GTGTATTATTGTGCGAGACTAGGGACCTATGGTGGCTACGATCCCCAGGG14810.16248691656664377No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG14770.16204805926329025No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGGAAG14330.15722062892640143No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT14240.15623319999385601No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13640.14965034044355308No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT13370.14668805364591678No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12920.14175090898318957No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12670.13900805083723No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12510.1372526216238159No Hit
ATACACAGCCGTGTCCGCGGCGGTCACAGAGCTCAGCCTCAGGGAGAATT11430.12540347443327066No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAG11380.12485490280407874No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT11140.12222175898395757No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACT11100.12178290168060404No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT10530.11552918510781628No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA10370.11377375589440215No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG10300.11300575561353349No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10180.11168918370347289No Hit
AGTTTAGAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGAC10080.11059204044508908No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA9910.10872689690583659No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG9820.10773946797329115No Hit
TTACAGTACCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC9790.107410324995776No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA9690.10631318173739217No Hit
GTTTAGAGAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACA9590.10521603847900836No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA9570.1049966098273316No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9500.10422860954646292No Hit
ATGTAGTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGGTGATAC9430.10346060926559424No Hit
CCTTCCCAGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG9360.10269260898472558No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9220.10115660842298822No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG9190.10082746544547308No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)