FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979846
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35880.18122621658452223No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC31910.16117415192898843No Hit
TTATAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGA26830.13551559060654214No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26510.13389930327914393No Hit
GACTTAGGACGGTCAACTTAGTCCCTCCGCCGAACACTCGATAATAGTCG26330.13299014165748244No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA26160.13213148901480215No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC25200.1272826270326076No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22650.11440283739240324No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA22640.11435232841342205No Hit
GTCTTATAATGGCGACTATTATCGAGTGTTCGGCGGAGGGACTAAGTTGA22510.11369571168666653No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20830.10521020321782604No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC20700.10455358649107051No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20580.1039474787432962No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT20150.10177559264710487No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36250.074.7222061
GGTATCA15900.061.618571
TCAACGC43950.059.8052374
ATCAACG44200.059.4669723
TATCAAC46850.056.868982
CAACGCA46800.056.314285
AACGCAG48350.054.6551326
CGCAGAG57300.046.115928
GCAGAGT65100.040.8117149
ACGCAGA81700.032.6957477
GTACATG53350.031.4623621
TACATGG54250.029.8652462
GTACACG15800.029.623725
TACACGG16050.029.1608186
CAGAGTA65400.029.0646829
ACATGGG55500.028.8048253
ACACGGG17850.027.0256427
CATGGGG41200.026.3927694
GTATGTA22150.025.0430261
GAATACG3551.6916601E-1024.3384179
Produced by FastQC (version 0.11.5)