FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106201
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTACTGTGCGAGCCTCGGTGGTGGGAATCGGGAGTACTGGGGCCA35920.3247149478259376No Hit
GTAATATACAGCCGTGTCCTCGACTCTCAGACTGTTCATTTGAAGAGACA34430.3112454246560978No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT32890.2973239040644512No Hit
GTCTACATCTGTAGGAGACAGAGTCACCATCACGTGCCAGGCGAGTCAGG27830.2515817649776126No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC26800.24227061808839442No Hit
CTATGTTACTGTCCCTCGTACTTTCGGCGGAGGGACCAAGGTGGAGATCA25960.23467706140204175No Hit
ATATTACTGTCAACACTATGTTACTGTCCCTCGTACTTTCGGCGGAGGGA21800.19707087590772385No Hit
ATGTTACTGTCCCTCGTACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA20610.18631333726872423No Hit
GTAACATAGTGTTGACAGTAATATGTTGCAATATCTTCAGGCTGCAGGCT19300.17447100481738853No Hit
GTAGGAGACAGAGTCACCATCACGTGCCAGGCGAGTCAGGACATTAGCAA17200.15548711310150687No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16380.1480743553838769No Hit
TATAAAAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT16300.14735115950898617No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15950.1441871775563392No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15940.14409677807197788No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15150.13695521880743192No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14930.1349664301514824No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14890.13460483221403705No Hit
ACATATTACTGTCAACACTATGTTACTGTCCCTCGTACTTTCGGCGGAGG14800.13379123685478497No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT14560.1316216492301128No Hit
GTCTGAGAGTCGAGGACACGGCTGTATATTACTGTGCGAGCCTCGGTGGT14400.13017525748033132No Hit
GCTGTATATTACTGTGCGAGCCTCGGTGGTGGGAATCGGGAGTACTGGGG14060.12710167501204572No Hit
ATATTACTGTGCGAGCCTCGGTGGTGGGAATCGGGAGTACTGGGGCCAGG14020.12674007707460036No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14020.12674007707460036No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13880.12547448429354158No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13730.12411849202812147No Hit
GTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAA13200.11932731935697038No Hit
GTCTCCATCCTCCCTGTCTACATCTGTAGGAGACAGAGTCACCATCACGT13120.11860412348207966No Hit
GTACATGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGT12990.11742893018538221No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12980.11733853070102088No Hit
ATATACAGCCGTGTCCTCGACTCTCAGACTGTTCATTTGAAGAGACAGTG12910.11670573431049149No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12870.11634413637304612No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG12650.11435534771709663No Hit
CAGTAATATGTTGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGT12610.11399374977965125No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11710.10585779618713055No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11430.103326610625013No Hit
TTACTGTCCCTCGTACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA11420.10323621114065167No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11290.10206101784395422No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11230.10151862093778619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC14950.057.7250754
GTATCAA15300.057.4226261
ATCAACG15750.054.7905273
TATATCG400.006245653.9417535
CAACGCA15950.053.6599245
AACGCAG17450.049.053996
TATCAAC17750.047.8066642
CGCAGAG19850.042.033998
GTACATG30400.036.006811
CAGCGCT5500.035.3105249
GCAGAGT24350.034.5612959
CAGCCGT16350.033.4348149
TATACAG18800.032.9006425
AATATAC17650.032.18753
CATGGGA18850.030.9028224
CTGTGCG23400.030.1240489
ATACAGC19400.030.0335086
ATATACA20950.028.8349864
GGCAGCG6750.028.7728427
TAATATA20150.027.8371262
Produced by FastQC (version 0.11.5)