FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027513
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG52980.5156139143738327No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTGATTGGGTGTT44650.43454438046039323No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC36370.35396145839517357No Hit
CATGTATACTGGTACCAGCACGTCCCAGGAACGGCCCCCAAACTCCTCAT32060.312015517078616No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATCACCACTC29140.28359738514257243No Hit
CTATACGAGTGATCAGCGGCCCTCCGGGGTCCCTGACCGATTCTCTGGCG28380.2762008850496296No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT27060.2633543322566235No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC26780.26062930590659195No Hit
GTAATACACGGCCGTGTCTTCGGATCTCAGGCTTCTCAGCTCCATGTAGA26320.2561524769029686No Hit
TCCCAGGAACGGCCCCCAAACTCCTCATCTATACGAGTGATCAGCGGCCC25070.2439871806974705No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT24150.23503352269022387No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA23300.22676112127048512No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA22840.22228429226686183No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC22730.221213746200778No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC21920.2133306342596152No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA21820.21235741056317534No Hit
GTCTACATGGAGCTGAGAAGCCTGAGATCCGAAGACACGGCCGTGTATTA20320.19775905511657763No Hit
GTTCCCCACTATGGACTGGATTTGGAGGATCCTCTTCTTGGTGGGAGCAG20100.19561796298440992No Hit
GTGTATTACTGTGCGGCAGATCAAGTCACGGTTTTTGGAGTGGTAATGAA19600.1907518445022107No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19130.1861776931289434No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA18650.1815062193860321No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC18380.1788785154056445No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT18060.17576419957703698No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGTGTCAC17950.17469365351095315No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17770.1729418508573614No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17430.16963289028946593No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA17390.16924360081089No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17380.169146278441246No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17250.16788108763587417No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16920.16466944943762268No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16690.16243103493581101No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT16590.16145781123937117No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATCACCACTCA16530.16087387702150727No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16530.16087387702150727No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA16240.15805152830183172No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16220.15785688356254374No Hit
GTCTGGGCCTGAGGTGAAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCA16020.15591043616966402No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC15940.15513185721251216No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT15850.15425595588571628No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15730.15308808744998847No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA15710.1528934427107005No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15410.14997377162138095No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15090.14685945579277343No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG15070.14666481105348544No Hit
CTCGTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACGTGCTGGTACCA14970.1456915873570456No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14710.14316120574630198No Hit
TTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTGATTGGGTGTTCG13490.13128787664973582No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC13360.13002268584436402No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG13090.1273949818639764No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13010.12661640290682455No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCGGACCGGAGCCCAC12930.12583782394967266No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12710.123696731817505No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12630.12291815286035311No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGA12590.12252886338177717No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC12590.12252886338177717No Hit
GTATTACTGTGCGGCAGATCAAGTCACGGTTTTTGGAGTGGTAATGAAGA12320.11990115940138957No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG12140.11814935674779783No Hit
CATCTATACGAGTGATCAGCGGCCCTCCGGGGTCCCTGACCGATTCTCTG12080.11756542252993392No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG12040.11717613305135799No Hit
GCATGGGATGACAGCCTGAGTGGTGATTGGGTGTTCGGCGGAGGGACCAA11850.11532700802812228No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC11650.11338056063524256No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11460.11153143561200686No Hit
CTCCTCATCTATACGAGTGATCAGCGGCCCTCCGGGGTCCCTGACCGATT11430.1112394685030749No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG11350.11046088954592302No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG11340.11036356717627903No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11310.11007160006734709No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT11310.11007160006734709No Hit
CTTCATTACCACTCCAAAAACCGTGACTTGATCTGCCGCACAGTAATACA11210.10909837637090723No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT11160.1086117645226873No Hit
GGATAGGATGGATCGTCGTTGGCAGTGGAAGCACAAAGTACGCACAGAAA11140.10841711978339934No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCC11090.1079305079351794No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA10940.10647067239051963No Hit
TCCTACCACTGCTGTGCAGTGGGTGCGACAGGCTCGTGGACAACGCCTTG10640.10355100130120008No Hit
TTACTGTGCAGCATGGGATGACAGCCTGAGTGGTGATTGGGTGTTCGGCG10620.10335635656191211No Hit
GTGTCACCATCTCTTGTTCTGGAGGCAGCTCCAACATCGGAAGTAATCAT10530.10248045523511624No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG10530.10248045523511624No Hit
CCCAATCACCACTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC10370.10092329732081248No Hit
GTAATGAAGAAGTCCTACTACTACGGAATGGACGTCTGGGGCCAAGGGAC10320.10043668547259257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6850.084.2634351
GTATCAA20950.068.190031
ATCAACG22800.061.939573
TATAACG350.003655157561.7589873
TCAACGC23000.061.4009674
CAACGCA23850.059.5147745
TATCAAC23900.059.088792
AACGCAG24700.057.4051746
CAGCGCT5300.051.6022159
CGCAGAG27900.051.0765538
ACGCAGA31500.045.4677167
TACGGAT803.89962E-544.9821937
GCAGCGC7100.038.5199628
GTACATG27700.035.945051
GGCAGCG7700.035.518417
ACATGGG27900.035.3804473
TATTGAG17050.032.9622735
GTACACG10150.031.9443045
TTATTGA18300.031.498214
CAGGGCA9200.030.5438484
Produced by FastQC (version 0.11.5)