FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027513
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTGATTGGGTGTT42560.41420400520480033No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG34460.3353728857931725No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC33640.3273924514823657No Hit
CATGTATACTGGTACCAGCACGTCCCAGGAACGGCCCCCAAACTCCTCAT31460.30617617489997695No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT29430.28641973386224795No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC27390.26656597045487507No Hit
CTATACGAGTGATCAGCGGCCCTCCGGGGTCCCTGACCGATTCTCTGGCG26240.2553738979458168No Hit
GTAATACACGGCCGTGTCTTCGGATCTCAGGCTTCTCAGCTCCATGTAGA25220.24544701624213028No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATCACCACTC24640.23980231880277914No Hit
TCCCAGGAACGGCCCCCAAACTCCTCATCTATACGAGTGATCAGCGGCCC24290.23639603586523963No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT22360.21761281852395054No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC22050.214595825064987No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA20760.20204123938091295No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA19930.19396348270046218No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC19780.19250364715580243No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19190.1867616273468073No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA19120.1860803707592994No Hit
GTCTACATGGAGCTGAGAAGCCTGAGATCCGAAGACACGGCCGTGTATTA18850.1834526667789118No Hit
GTGTATTACTGTGCGGCAGATCAAGTCACGGTTTTTGGAGTGGTAATGAA18040.17556955483774903No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGTGTCAC17910.1743043640323772No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT17650.1717739824216336No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17120.16661589683050237No Hit
GTTCCCCACTATGGACTGGATTTGGAGGATCCTCTTCTTGGTGGGAGCAG16690.16243103493581101No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC16670.16223639019652306No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG16510.16067923228221928No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16450.16009529806435538No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA16250.1581488506714757No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA15710.1528934427107005No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15510.1509469953178208No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15330.14919519266422906No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15250.1484166137070772No Hit
CTCGTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGACGTGCTGGTACCA14660.14267459389808207No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA14650.14257727152843808No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14590.14199333731057417No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA14460.14072814650520235No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14350.13965760043911854No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATCACCACTCA14320.1393656333301866No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT14260.13878169911232266No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14120.13741918593730687No Hit
GTCTGGGCCTGAGGTGAAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCA14100.1372245411980189No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC13850.13479148195691928No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13790.13420754773905536No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT13550.13187181086759972No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13150.1279789160818403No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13010.12661640290682455No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12900.12554585684074068No Hit
GTATACATGATTACTTCCGATGTTGGAGCTGCCTCCAGAACAAGAGATGG12800.12457263314430084No Hit
TTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTGATTGGGTGTTCG12560.1222368962728452No Hit
CTCCTCATCTATACGAGTGATCAGCGGCCCTCCGGGGTCCCTGACCGATT12520.12184760679426927No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12480.12145831731569331No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC12370.1203877712496095No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG12260.11931722518352565No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC12080.11756542252993392No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG12020.11698148831207002No Hit
GTATTACTGTGCGGCAGATCAAGTCACGGTTTTTGGAGTGGTAATGAAGA11860.11542433039776626No Hit
CATCTATACGAGTGATCAGCGGCCCTCCGGGGTCCCTGACCGATTCTCTG11640.1132832382655986No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGA11620.11308859352631062No Hit
GCATGGGATGACAGCCTGAGTGGTGATTGGGTGTTCGGCGGAGGGACCAA11300.1099742776977031No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11260.10958498821912716No Hit
CTTCATTACCACTCCAAAAACCGTGACTTGATCTGCCGCACAGTAATACA11250.10948766584948316No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10730.10442690262799595No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10690.10403761314942No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10590.10306438945298015No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10580.10296706708333617No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG10460.10179919864760835No Hit
GTGTCACCATCTCTTGTTCTGGAGGCAGCTCCAACATCGGAAGTAATCAT10310.10033936310294858No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)