FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2419766
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGCTGTCAGTGTC84470.34908334111645506No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC61470.254032827967663No Hit
GCCTTGGGCTGACCCAGGACGGTCAGTTTCGTCCCTCCGCCGAATATCTT54200.22398860055063174No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45340.18737348983331445No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43980.18175311166451633No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA41320.1707603131873082No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC37680.15571753632376023No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA36820.1521634736581967No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT35900.148361453132245No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT35190.14542728511765188No Hit
TTATAAGATATTCGGCGGAGGGACGAAACTGACCGTCCTGGGTCAGCCCA33980.14042680159982412No Hit
ATCTTATAATTACCAGTGGGGTCCGTTGAGTAACAGTAGAAGTCAGCTTC33410.13807120192613667No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA33400.13802987561607197No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC32690.13509570760147882No Hit
CCTATGAGCTGACACAGCCACCCTCGCTGTCAGTGTCCCCAGGACAAACG30260.1250534142557586No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29450.12170598314051856No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTG28940.11959834132721923No Hit
TATCAGGACAGTAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTC28780.11893712036618417No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA28560.11802794154476094No Hit
CCCCTGTGCTGGTCATCTATCAGGACAGTAAACGACCCTCCGGGATCCCT27880.11521775246036187No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG27460.1134820474376448No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA27250.11261419492628626No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27240.11257286861622157No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG27200.1124075633759628No Hit
GTAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGG27000.11158103717466895No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG26850.11096114252369857No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG26480.10943206905130497No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA26290.10864686916007582No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26070.10773769033865259No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGCTGTCAGTGTCCC25650.10600198531593552No Hit
GACTATTAGTGGGGCCCAGGCGGAAGATGAAGCTGACTTCTACTGTTACT24570.10153874382894876No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22350.076.706521
GTATCAA60900.073.925721
TCAACGC68600.064.0303654
ATCAACG68750.063.9953963
TATCAAC74250.059.447732
CAACGCA74500.058.9558455
AACGCAG75900.057.867196
ACGCAGA82750.053.0758827
CGCAGAG86050.051.040438
GCAGAGT102950.042.661779
GTACATG79450.033.7273251
TATTGAG40550.031.7845065
GAATACG6900.031.3045549
TACATGG83000.031.0581912
ACATGGG86050.030.2090133
TTATTGA44200.028.5099224
GTGCTAG9250.027.2558161
CATGGGG52350.027.2350854
CAGAGTA97750.026.148519
ATTGAGT29650.024.7696466
Produced by FastQC (version 0.11.5)