FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2419766
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGCTGTCAGTGTC76550.31635290354521883No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC50960.21059887608967146No Hit
GCCTTGGGCTGACCCAGGACGGTCAGTTTCGTCCCTCCGCCGAATATCTT47900.19795302520987565No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44420.1835714693073628No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38180.15778385182699484No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA37770.15608947311434246No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT33930.14022017004950066No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32220.1331533710284383No Hit
ATCTTATAATTACCAGTGGGGTCCGTTGAGTAACAGTAGAAGTCAGCTTC31670.13088042397488023No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA31000.12811156120054584No Hit
TTATAAGATATTCGGCGGAGGGACGAAACTGACCGTCCTGGGTCAGCCCA30280.12513606687588802No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC30050.12418556174440007No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT29890.12352434078336501No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC28810.11906109929637826No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27720.11455653149932679No Hit
CCTATGAGCTGACACAGCCACCCTCGCTGTCAGTGTCCCCAGGACAAACG26180.1081922797493642No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA26110.10790299557891135No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA25550.10558872221528859No Hit
CCCCTGTGCTGGTCATCTATCAGGACAGTAAACGACCCTCCGGGATCCCT25380.10488617494418882No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA25290.1045142381536066No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG25200.10414230136302435No Hit
TATCAGGACAGTAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTC25180.10405964874289499No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG25140.1038943435026362No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG24360.10067089131759022No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG24330.10054691238739613No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56450.074.482091
GGTATCA22200.071.99511
TCAACGC66550.062.7533234
ATCAACG66750.062.6731723
TATCAAC70500.059.3394932
CAACGCA77700.053.8408555
AACGCAG79850.052.3009876
ACGCAGA79950.051.9653857
CGCAGAG82050.050.5476238
GCAGAGT97850.042.084319
GTACATG74850.029.2404961
GAATACG7350.028.4050589
TACATGG77200.028.2606492
ACATGGG79450.027.3696883
TATTGAG38000.027.0962545
CATGGGG47550.026.197054
CAGAGTA91700.025.279689
TTATTGA41500.024.984534
GTGCTAG9750.024.3675731
GCCCATA10350.024.346171
Produced by FastQC (version 0.11.5)