FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619018
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAATAGTGTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA27740.44812913356315975No Hit
CTATTATATTGTTGACAGTAAAATGTTCCAACATCTTCAGCCTGCAGGCC21220.3428010170948179No Hit
GTAATAGAGGGCCGTGTCACCAGATCTTAGGCTGCTCAGGTCCAGGAACA20600.3327851532588713No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCTTCGGTGAGGGTCTCCTGCA16530.26703585356160886No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT16510.26671276117980414No Hit
CTCTATTACTGTGCGCGACACTTCAGGCGCTATAGTGATACTGCCCCCTT15680.2533044273349079No Hit
CTATTACTGTGCGCGACACTTCAGGCGCTATAGTGATACTGCCCCCTTTG13840.22357992820887274No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12010.19401697527374No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11690.18884749716486435No Hit
ATTATATTGTTGACAGTAAAATGTTCCAACATCTTCAGCCTGCAGGCCGT11590.1872320352558407No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC11300.18254719571967212No Hit
CAGTAATAGAGGGCCGTGTCACCAGATCTTAGGCTGCTCAGGTCCAGGAA11200.18093173381064848No Hit
GTATCACTATAGCGCCTGAAGTGTCGCGCACAGTAATAGAGGGCCGTGTC10440.16865422330206878No Hit
TAGTGATACTGCCCCCTTTGACTCCTGGGGCCAGGGAACCCTGGTCACCG10410.16816958472936167No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9770.15783062851161034No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9720.1570228975570985No Hit
ATAGTGATACTGCCCCCTTTGACTCCTGGGGCCAGGGAACCCTGGTCACC9460.152822696593637No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9250.14943022658468738No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9200.14862249563017554No Hit
CTATAGTGATACTGCCCCCTTTGACTCCTGGGGCCAGGGAACCCTGGTCA9200.14862249563017554No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9060.14636084895754245No Hit
CAATATAATAGTGTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGAT9000.14539157181212822No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAGACATAAGTTA8970.14490693323942114No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8780.1418375556122762No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8730.1410298246577644No Hit
TCACTATAGCGCCTGAAGTGTCGCGCACAGTAATAGAGGGCCGTGTCACC8700.14054518608505728No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA8540.13796044703061947No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8440.13634498512159582No Hit
GCCTAAGATCTGGTGACACGGCCCTCTATTACTGTGCGCGACACTTCAGG8100.1308524146309154No Hit
ATCTTAGGCTGCTCAGGTCCAGGAACACAGTGCTCGTGGACTCGTCCGCG7570.12229046651309008No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG7460.12051345841316408No Hit
ATAGTGTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA7280.11760562697692152No Hit
CTACAGGACCCCTGGGACCACAGCTCATCACCATGGACTGGACCTGGAGG7060.11405161077706948No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6970.1125976950589482No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6740.10888213266819383No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA6740.10888213266819383No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6740.10888213266819383No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT6670.10775130933187727No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT6630.10710512456826782No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT6620.10694357837736544No Hit
ACATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG6460.1043588393229276No Hit
CATAACAACCACATTCCTCCTCTACAGGACCCCTGGGACCACAGCTCATC6430.10387420075022051No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT6390.10322801598661106No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA6230.10064327693217322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC16900.060.954514
ATCAACG17000.060.5959553
CAACGCA17400.059.1981545
AACGCAG18200.056.945786
CGCAGAG20300.051.054848
ACGCAGA21950.047.2170077
CAGCGCT2900.044.672999
GTATCAA25700.040.9867861
GAATACG2800.038.557049
TATCAAC27100.038.278042
GCAGCGC3600.037.985838
GGTATCA15600.037.461451
GCAGAGT30250.034.4995239
CTAGAGA4150.032.980864
GGCAGCG3800.032.198517
TAATAGT13650.032.1924254
ATAGTGT13900.032.1031276
TAGTGTC13850.031.6993627
GTACATG16300.031.4264181
AATAGTG14250.031.3399435
Produced by FastQC (version 0.11.5)