FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences619018
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAATAGTGTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA28700.4636375678897868No Hit
CTATTATATTGTTGACAGTAAAATGTTCCAACATCTTCAGCCTGCAGGCC20700.33440061516789493No Hit
GTAATAGAGGGCCGTGTCACCAGATCTTAGGCTGCTCAGGTCCAGGAACA19650.31743826512314666No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCTTCGGTGAGGGTCTCCTGCA16130.2605740059255143No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT15300.247165672080618No Hit
CTCTATTACTGTGCGCGACACTTCAGGCGCTATAGTGATACTGCCCCCTT15190.24538866398069198No Hit
CTATTACTGTGCGCGACACTTCAGGCGCTATAGTGATACTGCCCCCTTTG13720.2216413739180444No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11240.18157791857425792No Hit
CAGTAATAGAGGGCCGTGTCACCAGATCTTAGGCTGCTCAGGTCCAGGAA11230.18141637238335556No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11140.1799624566652343No Hit
ATTATATTGTTGACAGTAAAATGTTCCAACATCTTCAGCCTGCAGGCCGT10930.17656998665628462No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC9790.15815372089341506No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9690.15653825898439142No Hit
GTATCACTATAGCGCCTGAAGTGTCGCGCACAGTAATAGAGGGCCGTGTC9680.15637671279348905No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9360.1512072346846134No Hit
TAGTGATACTGCCCCCTTTGACTCCTGGGGCCAGGGAACCCTGGTCACCG9300.15023795753919916No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA8990.1452300256212259No Hit
CAATATAATAGTGTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGAT8690.14038363989415495No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8660.13989900132144784No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8540.13796044703061947No Hit
CTATAGTGATACTGCCCCCTTTGACTCCTGGGGCCAGGGAACCCTGGTCA8280.133760246067158No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8140.13149859939452488No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAAGACATAAGTTA8120.13117550701272016No Hit
TCACTATAGCGCCTGAAGTGTCGCGCACAGTAATAGAGGGCCGTGTCACC8100.1308524146309154No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8000.12923695272189176No Hit
ATAGTGATACTGCCCCCTTTGACTCCTGGGGCCAGGGAACCCTGGTCACC7950.12842922176737995No Hit
GCCTAAGATCTGGTGACACGGCCCTCTATTACTGTGCGCGACACTTCAGG7480.1208365507949688No Hit
ATAGTGTCCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA7440.12019036603135935No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7190.11615171125880022No Hit
ATCTTAGGCTGCTCAGGTCCAGGAACACAGTGCTCGTGGACTCGTCCGCG7100.11469779554067895No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT7030.11356697220436239No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG6800.10985140981360801No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6700.10823594790458435No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT6680.10791285552277961No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6610.10678203218646308No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT6470.10452038551382997No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA6290.1016125540775874No Hit
CTACAGGACCCCTGGGACCACAGCTCATCACCATGGACTGGACCTGGAGG6230.10064327693217322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG9150.057.348023
CAACGCA9100.056.8870125
TCAACGC9200.056.2686774
AACGCAG9800.053.5746546
GAATACG1800.047.9480939
CGCAGAG11550.045.4572878
ACGCAGA13700.038.8484577
GTGCTAG2451.8189894E-1235.2328761
ATAATAG14500.034.205643
AGTGTCC15100.033.81778
TAGTGTC14250.033.8160257
AATAGTG14750.033.6340455
TAATAGT14600.033.487144
ATAGTGT14500.033.232996
GTACATG16050.032.2693631
CAGCGCT3600.031.9653979
CTATTAT9700.031.8881721
TATAATA16600.031.6105062
TATTATA11400.030.8963992
TATGTAC5150.030.7066272
Produced by FastQC (version 0.11.5)