FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1736178
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT42940.24732487106736753No Hit
ATCTATTACTGTGCGAGAGGGGGGGATGGCTATTCTCTTGACAATTGGGG41320.23799403056599036No Hit
GTTTAATAATTACCTGGTCACCTTCGGCCAAGGGACACGACTGGAGATTA40240.23177347023173892No Hit
TTATTAAACTGTTGACAATAATAAGTTGCAAAATCTTCAGGCTGCAGGCT37410.21547329824476522No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36120.20804318451218712No Hit
GTAATAGATAGCCGTGTCCTCGACTCTCAGTCTGCCCATTTGCAAATACA34000.19583245496717505No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30530.17584602500434862No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT30360.17486686272951277No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCC27830.16029462416872003No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26060.15009981695425237No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG25640.14768071015759907No Hit
GTCGGAGACAGAGTCATCATCACTTGCCGGGCAAGTCAGGGCATTGACAG23710.13656433844916824No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCATCATCACTT22530.12976780030618978No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22520.12971020252531712No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22270.12827025800349964No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21710.1250447822746285No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21630.12458400002764694No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG21550.12412321778066535No Hit
GTCTCGAATAGAGCTCTTGGAAGTCCCTCCAACCATGGCCTGGGTCTCCT19910.11467718171754278No Hit
ATAATTACCTGGTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGA19490.11225807492088946No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG19440.11197008601652594No Hit
GGTAATTATTAAACTGTTGACAATAATAAGTTGCAAAATCTTCAGGCTGC19160.11035734815209039No Hit
GCTTTAGTCTGGTATCAGCAGAAACCAGGGAGAGCTCCTAAGCTCTTGAT18910.10891740363027293No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18890.10880220806852754No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT18230.10500075453092944No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT17770.1023512566107853No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38250.075.332551
ATCAACG44150.064.7461243
TCAACGC44950.063.273434
CAACGCA48150.059.068345
AACGCAG50200.056.797986
CGCAGAG56200.050.6045658
ACGCAGA59450.047.9592367
GCAGAGT62250.046.033369
TATCAAC66400.044.1347122
TAGCCGT14250.041.431249
GTAATAG16350.037.8908921
GATAGCC16400.037.316777
TAGATAG17200.036.4204035
TAATAGA17700.034.577982
GTATACG1251.3224253E-534.577641
CTGTGCG19000.032.5892079
GTACATG50300.032.2231981
TACATGG51350.031.5497572
ACATGGG51150.031.532353
CATGGGA22900.031.1281784
Produced by FastQC (version 0.11.5)