FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1736178
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATTACTGTGCGAGAGGGGGGGATGGCTATTCTCTTGACAATTGGGG39770.2290663745307221No Hit
GTTTAATAATTACCTGGTCACCTTCGGCCAAGGGACACGACTGGAGATTA39520.22762643000890462No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT38240.22025391405719918No Hit
TTATTAAACTGTTGACAATAATAAGTTGCAAAATCTTCAGGCTGCAGGCT37620.2166828516430919No Hit
GTAATAGATAGCCGTGTCCTCGACTCTCAGTCTGCCCATTTGCAAATACA32130.1850616699439804No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30200.17394529823554958No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28510.16421127326806353No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT27610.15902747298952066No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCC25380.1461831678549089No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23870.1374859029431314No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22120.12740629129040915No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21530.12400802221891995No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21290.12262567547797519No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG20740.11945779752997676No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19180.1104725437138358No Hit
GTCGGAGACAGAGTCATCATCACTTGCCGGGCAAGTCAGGGCATTGACAG19080.10989656590510881No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG18490.10649829683361958No Hit
GGTAATTATTAAACTGTTGACAATAATAAGTTGCAAAATCTTCAGGCTGC18380.10586472124401991No Hit
ATAATTACCTGGTCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGA18260.10517354787354753No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCATCATCACTT17730.10212086548729452No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17690.10189047436380372No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)