FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1328027
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGCTGTCAGTGTC65710.4947941570465058No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC46760.35210127504937777No Hit
GCCTTGGGCTGACCCAGGACGGTCAGTTTCGTCCCTCCGCCGAATATCTT42000.31625863028387224No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30130.22687791739173976No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA27560.20752590120532188No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT27240.20511631164125427No Hit
ATCTTATAATTACCAGTGGGGTCCGTTGAGTAACAGTAGAAGTCAGCTTC26590.20022183283924197No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA25720.1936707612119332No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25660.19321896316867052No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA25360.19095997295235714No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24890.18742088828013287No Hit
TTATAAGATATTCGGCGGAGGGACGAAACTGACCGTCCTGGGTCAGCCCA24590.18516189806381947No Hit
CCTATGAGCTGACACAGCCACCCTCGCTGTCAGTGTCCCCAGGACAAACG23740.17876142578426493No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC22910.17251155285246458No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC22900.1724362531785875No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG22710.17100555937492234No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTG22160.16686407731168115No Hit
CCCCTGTGCTGGTCATCTATCAGGACAGTAAACGACCCTCCGGGATCCCT21770.16392739003047377No Hit
TATCAGGACAGTAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTC21640.1629484942700713No Hit
GTAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGG20370.15338543568767804No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20130.15157824351462734No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG20030.1508252467758562No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA19920.14999695036320798No Hit
CTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGCTGTCAGTGTCCC19650.14796385916852595No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA19390.146006067647721No Hit
GTCCCTGAGCTGGAGCCAGAGAATCTCTCAGGGATCCCGGAGGGTCGTTT19270.14510247156119566No Hit
CTAATAGTCAAGGTGGCCACTGTCCCTGAGCTGGAGCCAGAGAATCTCTC18910.14239168330161964No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG18840.14186458558447984No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCG18780.14141278754121717No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18680.14065979080244603No Hit
GACTATTAGTGGGGCCCAGGCGGAAGATGAAGCTGACTTCTACTGTTACT18650.1404338917808147No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG18550.13968089504204356No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18090.1362171100436964No Hit
GTCCTGATAGATGACCAGCACAGGGGCCTGGCCTGACTTCTGCTGGTACC18060.13599121102206504No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG17440.13132263124168408No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT17120.1289130416776165No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT17010.12808474526496827No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17000.12800944559109115No Hit
GCTCTGGAGATGCATTGCCAAAAAAATATGCTTATTGGTACCAGCAGAAG16640.1252986573315151No Hit
CAGTAGAAGTCAGCTTCATCTTCCGCCTGGGCCCCACTAATAGTCAAGGT16460.12394326320172708No Hit
GACCCAGGACGGTCAGTTTCGTCCCTCCGCCGAATATCTTATAATTACCA16420.12364206450621862No Hit
CTTCTACTGTTACTCAACGGACCCCACTGGTAATTATAAGATATTCGGCG15630.11769339026992673No Hit
GTATCAACGCAGAGTACATGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAA15320.11535910037973626No Hit
ACCCAGGACGGTCAGTTTCGTCCCTCCGCCGAATATCTTATAATTACCAG15180.11430490494545668No Hit
CTATTAGTGGGGCCCAGGCGGAAGATGAAGCTGACTTCTACTGTTACTCA15110.11377780722831689No Hit
GTACATGGGGTGGGCTCAGGAGGCAGAGCTCTGGGAATCTCACCATGGCC15030.11317540983729998No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC14880.1120459147291433No Hit
GTGCTGGTCATCTATCAGGACAGTAAACGACCCTCCGGGATCCCTGAGAG14800.1114435173381264No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA14790.11136821766424929No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC14460.10888332842630458No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGTGAGGGTTGAG14130.10639843918835987No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13990.1053442437540803No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG13990.1053442437540803No Hit
GCTCAGGGACAGTGGCCACCTTGACTATTAGTGGGGCCCAGGCGGAAGAT13960.10511834473244895No Hit
GGTAATTATAAGATATTCGGCGGAGGGACGAAACTGACCGTCCTGGGTCA13670.1029346541900127No Hit
GCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTT13580.10225695712511869No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13510.1017298594079789No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC13400.10090156299533065No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11100.081.087991
GTATCAA28800.080.756891
ATCAACG35900.064.178913
TATCAAC36250.063.559252
TCAACGC36800.062.609324
CAACGCA41150.056.340785
AACGCAG42550.054.6562466
CGCAGAG45050.050.8240368
ACGCAGA45900.049.7259867
GCAGAGT57050.040.1305929
GAATACG3650.033.5320559
GTACATG35700.030.052981
TATACCG1255.3674774E-428.8002875
ACATGGG36350.028.7210583
CATGGGG25450.027.7252254
TCTACAC10500.027.4288443
GTGCTAG5350.026.9181821
TATTGAG22750.026.9013655
GGGTTTA14800.026.272511
CAGAGTA50550.026.2060039
Produced by FastQC (version 0.11.5)