FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850772
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC92140.49784630413686826No Hit
GTAATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTAGCAGATACA82870.44775909728480867No Hit
GTGTATTACTGTGCGAAAGATCTCCGATACAGTTATGGTTACGGGGCTAT70580.3813543753633619No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAGAGTGTTGGTG65190.35223139316998525No Hit
GTCCTGGGCCCACTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC58020.3134908027569036No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC56510.30533204522220997No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAG54830.29625475207102764No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTT53670.2899870972761637No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT48290.26091814658963935No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA43050.23260563699904688No Hit
GGTTATAACTATGTCTCCTGGTACCAACAAACCCCAGGCAAAGCCCCCAA42400.22909358905364896No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA42160.22779683288919436No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT41340.22336624932730773No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG40840.22066467398469394No Hit
CTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATAACTATGTCTCCT40350.21801713014893248No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA40190.21715262603929603No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC36640.19797144110673814No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTTCTGCAGCTCATAT36630.19791740959988588No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT36450.19694484247654492No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT35410.19132556576390825No Hit
TCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGCCCACTCTGCCCTG34310.18538210001015792No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33780.1825184301469873No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA33350.18019507535233945No Hit
GTATATGAGCTGCAGAAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA32540.17581852329730513No Hit
GCTCATATACAAGCACCAACACTCTGGTGTTCGGCGGAGGGACCAAACTG32500.17560239726989602No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT32070.17327904247524817No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC31770.1716580972696799No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG31610.1707935931600435No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA31300.16911861644762294No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC31220.16868636439280474No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC31110.1680920178174297No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG30730.16603882055704322No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCAGAGTGTTGGTGC30310.16376949726924764No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG30160.1629590246664635No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29670.16031148083070199No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA29540.1596090712416224No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG29020.15679943288530407No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA28830.15577283425511082No Hit
TCCTCACTCAGGTCACAGGGTCCTGGGCCCACTCTGCCCTGACTCAGCCT28550.1542599520632471No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA28450.15371963699472435No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGTCACAGGGTCCTGGGCCCACTC28320.1530172274056448No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG28090.15177450274804244No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC28020.15139628220007653No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG27750.14993743151506508No Hit
TATATGAGCTGCAGAAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAG27670.14950517946024686No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA27510.14864067535061046No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT27420.14815439178893997No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT26260.14188673699407597No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAATGACGTTGGTGGTTATA25870.13977950822683724No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC25790.13934725617201904No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGTCACAGGGTCCT25420.13734809041848484No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG24700.13345782192512098No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA24700.13345782192512098No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT24160.1305401205550981No Hit
CTCCTGTGTAGCCTCTGCATTCACCTTCAGTAGCTATGGCATGCACTGGG23830.128757080828973No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC23820.1287030493221207No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA23800.12859498630841618No Hit
GTGTAGCCTCTGCATTCACCTTCAGTAGCTATGGCATGCACTGGGTCCGC23550.12724419863710926No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC23180.12524503288357508No Hit
GTATTACTGTGCGAAAGATCTCCGATACAGTTATGGTTACGGGGCTATCT22780.12308377260948403No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG21690.117194338362586No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT21280.11497904658164268No Hit
ATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTAGCAGATACACCA21270.1149250150747904No Hit
GATAAATACTTTGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA21220.11465485754052904No Hit
GGTCACAGGGTCCTGGGCCCACTCTGCCCTGACTCAGCCTGCCTCCGTGT21030.11362825891033579No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG20970.11330406986922213No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20930.11308794384181303No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT20690.11179118767735842No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCAGCACTAGAAGTCGGCGGTGTT20450.11049443151290379No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG20370.1100621794580856No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG20330.1098460534306765No Hit
GTTATAACCACCAACGTCATTGCTGGTTCCAGTGCAGGAGATGGTGATCG20120.1087113917867787No Hit
ATCTGGGATTTACTCTACTACGGTATGGACGTCTGGGGCCAAGGGACCAC20080.1084952657593696No Hit
GTGTTGGTGCTTGTATATGAGCTGCAGAAATAATCAGCCTCGTCCTCAGC19850.10725254110176725No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG19700.10644206849898313No Hit
GCTGTGTATTACTGTGCGAAAGATCTCCGATACAGTTATGGTTACGGGGC19700.10644206849898313No Hit
GGGCTGGAGTGGGTGGCAGTTATATCATATGATGGAAGTGATAAATACTT18940.10233567397821017No Hit
TCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGTC18900.10211954795080107No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA18850.10184939041653969No Hit
GTTATGGTTACGGGGCTATCTGGGATTTACTCTACTACGGTATGGACGTC18790.10152520137542605No Hit
GTTCATTAGCAGATACACCATGTTCTTGGAATTGTCTCTGGAGATGGTGA18700.10103891781375554No Hit
CAGTAATACACAGCCGTGTCCTCAGGTCTCAGGCTGTTCATTAGCAGATA18700.10103891781375554No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18660.10082279178634646No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12500.086.461121
GTATCAA32500.078.9234851
ATCAACG39450.065.1597063
TCAACGC39800.064.767624
TATCAAC40450.063.9048582
CAACGCA42200.060.911875
AACGCAG43850.058.6166956
CGCAGAG50600.050.6549848
ACGCAGA58850.043.6761637
GCAGAGT68300.037.5277029
TATTGAG33050.036.1645285
TTATTGA34550.035.0121844
CATGGGA16150.032.9926454
ATTGAGG14800.031.620916
TACATGG46650.031.3330232
GTACATG47050.031.0868031
AGAGTCG950.004890346430.3167445
ACATGGG47900.030.2147123
TTGAGGG15150.029.939927
ATTGAGT24050.029.9369586
Produced by FastQC (version 0.11.5)