FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804362
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGACGTCAGCTTCAGGGAGA36380.45228392191575434No Hit
GTCTATTACTGTGCGAGAGATTCCTGGTTTGCGTTAGTCCCACCTCCTAA27440.3411399345071No Hit
GTCTCTGGTGACTCCATCCCCTGGAATGCTTACTACTGGGCCTGGATCCG25030.31117830031751875No Hit
CTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTCCTTG24330.3024757509678478No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC22150.2753735258503012No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACATGGTTTCCA18200.22626628309144392No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCCGGATCAC16640.20687203025503442No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT15530.1930722734291277No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA15240.1894669315556926No Hit
GTCATGGACCTCCTGTGCAAGAACATGAGGCACCTGTGGTTCTTCCTCCT14620.1817589592745555No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC14420.17927251660322094No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC14000.1740509869934184No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13910.17293208779131786No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13510.16795920244864873No Hit
TTCCAGTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCGCATCTTCCGCC12670.15751614322904364No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12400.154159445622742No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12170.15130003655070728No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12070.15005681521504No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11980.14893791601293943No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11830.14707308400943853No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA11830.14707308400943853No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT11750.1460785069409047No Hit
GCTTACTACTGGGCCTGGATCCGCCAGCCCCCAGGGAAGGGGTTGGAGTG11710.14558121840663782No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11530.14334342000243672No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11520.14321909786887No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11370.14135426586536906No Hit
AGCCAGGACAGGCCCCTGTCCTTGTCAATTATGGTAAATATAGCCGGCCC11260.13998672239613508No Hit
GGGACAGACAGTCCGGATCACATGCCAGGGAGACGGCCTCAGACCCTATT11200.1392407895947347No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11190.139116467461168No Hit
GTCTCTTATAATGGAAATAGCTACAACAACCCGTCCCTCAAGACTCGAGT11190.139116467461168No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11180.13899214532760126No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11160.1387435010604678No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC11030.13712731332410033No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCAC11010.13687866905696688No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT10940.1360084141219998No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTG10820.13451654851919906No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10780.13401925998493214No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA10760.13377061571779872No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA10750.13364629358423197No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10650.1324030722485647No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC10630.13215442798143123No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10600.13178146158073106No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10340.12854908610799615No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG10290.1279274754401625No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG10250.1274301869058956No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA10230.12718154263876216No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT10160.12631128770379504No Hit
GTACATGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCA10090.12544103276882795No Hit
CTATTACTGTAACTCCCGGGACAGCACTGGAAACCATGTGATATTCGGCG10030.1246950999674276No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9950.12370052289889379No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9870.12270594583035997No Hit
GGATTGGGAGTGTCTCTTATAATGGAAATAGCTACAACAACCCGTCCCTC9840.12233297942965979No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT9770.1214627244946927No Hit
AGCTACAACAACCCGTCCCTCAAGACTCGAGTCGCCATATCAGTTGACAC9740.12108975809399251No Hit
GTCTGTGGCCTTGGGACAGACAGTCCGGATCACATGCCAGGGAGACGGCC9690.12046814742615887No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA9580.11910060395692486No Hit
GTAATAGTCAGCCGCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGAAAG9510.11823034902195778No Hit
GACTATTACTGTAACTCCCGGGACAGCACTGGAAACCATGTGATATTCGG9480.1178573826212576No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9350.11624119488489013No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC9340.1161168727513234No Hit
GCACTGGAAACCATGTGATATTCGGCGGAGGGACCAAGCTGACCGTCCTA9310.11574390635062323No Hit
GTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTG9310.11574390635062323No Hit
CACTTGCACTGTCTCTGGTGACTCCATCCCCTGGAATGCTTACTACTGGG9310.11574390635062323No Hit
GTCCGGATCACATGCCAGGGAGACGGCCTCAGACCCTATTATGCAAGCTG9260.11512229568278959No Hit
TTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCTGTGT9260.11512229568278959No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9240.11487365141565613No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9100.11313314154572196No Hit
GATTGGGAGTGTCTCTTATAATGGAAATAGCTACAACAACCCGTCCCTCA8860.11014941034012049No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC8510.10579813566528504No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG8490.10554949139815158No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA8340.10368465939465067No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG8280.10293872659325032No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC8270.10281440445968357No Hit
CCCCTGTCCTTGTCAATTATGGTAAATATAGCCGGCCCCCAGGGATCGCA8130.1010738945897494No Hit
GTATCAACGCAGAGTACATGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGG8110.10082525032261594No Hit
GGTAAATATAGCCGGCCCCCAGGGATCGCAGACCGATTCTCTGGCTCCAG8110.10082525032261594No Hit
TCCTTGAACAGCGCTCTTCCCACTTGAGGGCGTCCAGGTGAAGGTGACAC8060.1002036396547823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5350.079.559671
GTATCAA18500.073.312981
ATCAACG19050.069.94693
TCAACGC19350.068.862464
CAACGCA19750.067.463585
TATCAAC20050.066.817522
AACGCAG20350.065.433786
ACGCAGA23250.057.2685857
CGCAGAG23600.056.1142928
ATAGACG13900.041.972224
TAGACGG14200.041.082935
GTGCTAG3450.039.730981
AATAGAC15150.038.984593
GCAGAGT35150.037.8803569
TAATAGA16350.037.0043952
GAATACG3150.036.5575529
CCGATCG800.002110660835.986349
TATTGAG11400.035.3791925
GTACATG23900.033.807641
TACATGG24100.033.472852
Produced by FastQC (version 0.11.5)