FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804362
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGACGTCAGCTTCAGGGAGA34960.4346301789492791No Hit
GTCTATTACTGTGCGAGAGATTCCTGGTTTGCGTTAGTCCCACCTCCTAA28550.35493969133300673No Hit
GTCTCTGGTGACTCCATCCCCTGGAATGCTTACTACTGGGCCTGGATCCG24320.30235142883428107No Hit
CTATTATGCAAGCTGGTACCAGCAGAAGCCAGGACAGGCCCCTGTCCTTG22760.2829571759978716No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC21860.27176818397686614No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCACATGGTTTCCA17410.21644483453967245No Hit
ACTCAGGACCCTGCTGTGTCTGTGGCCTTGGGACAGACAGTCCGGATCAC14740.18325082487735622No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA14510.18039141580532148No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA14130.17566717472978582No Hit
GTCATGGACCTCCTGTGCAAGAACATGAGGCACCTGTGGTTCTTCCTCCT13660.16982403445214966No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC13660.16982403445214966No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC13540.16833216884934893No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12810.15925665309897782No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT12640.15714317682834344No Hit
TTCCAGTGCTGTCCCGGGAGTTACAGTAATAGTCAGCCGCATCTTCCGCC12620.15689453256121No Hit
GTCTCTTATAATGGAAATAGCTACAACAACCCGTCCCTCAAGACTCGAGT11570.1438407085367036No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11560.1437163864031369No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11410.14185155439963598No Hit
GCTTACTACTGGGCCTGGATCCGCCAGCCCCCAGGGAAGGGGTTGGAGTG11390.14160291013250254No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11230.1396137559954349No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11120.1382462125262009No Hit
CTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGCT10950.13613273625556652No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10940.1360084141219998No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10830.13464087065276578No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA10680.13277603864926488No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10500.13053824024506377No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAATATCAC10490.13041391811149705No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA10450.12991662957723013No Hit
AGCCAGGACAGGCCCCTGTCCTTGTCAATTATGGTAAATATAGCCGGCCC10390.1291706967758298No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGTTCTG9920.1233275564981936No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9910.12320323436462687No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9840.12233297942965979No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA9790.12171136876182614No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC9720.12084111382685905No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG9690.12046814742615887No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9660.1200951810254587No Hit
GGGACAGACAGTCCGGATCACATGCCAGGGAGACGGCCTCAGACCCTATT9580.11910060395692486No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9560.11885195968979143No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT9490.11798170475482432No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA9340.1161168727513234No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG9320.11586822848418996No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG9260.11512229568278959No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9260.11512229568278959No Hit
GGATTGGGAGTGTCTCTTATAATGGAAATAGCTACAACAACCCGTCCCTC9230.11474932928208942No Hit
CTATTACTGTAACTCCCGGGACAGCACTGGAAACCATGTGATATTCGGCG9220.11462500714852267No Hit
GACTATTACTGTAACTCCCGGGACAGCACTGGAAACCATGTGATATTCGG9200.11437636288138923No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA9200.11437636288138923No Hit
GCACTGGAAACCATGTGATATTCGGCGGAGGGACCAAGCTGACCGTCCTA9150.11375475221355559No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA9080.1128844972785885No Hit
GTACATGGGAGCTGTGGGCTCAGAAGCAGAGTTCTGGGGTGTCTCCACCA9040.11238720874432158No Hit
CTCCTCACTCTTTGCATAGGTTCTGTGGTTTCTTCTGAGCTGACTCAGGA9000.11188992021005469No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8970.1115169538093545No Hit
GTCCGGATCACATGCCAGGGAGACGGCCTCAGACCCTATTATGCAAGCTG8930.11101966527508758No Hit
GTCTGTGGCCTTGGGACAGACAGTCCGGATCACATGCCAGGGAGACGGCC8910.11077102100795412No Hit
GTAATAGTCAGCCGCATCTTCCGCCTGAGCCCCAGTGATGGTCAAGAAAG8860.11014941034012049No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG8750.10878186687088649No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG8730.10853322260375303No Hit
AGCTACAACAACCCGTCCCTCAAGACTCGAGTCGCCATATCAGTTGACAC8620.10716567913451903No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC8560.10641974633311868No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8480.10542516926458484No Hit
CCCCTGTCCTTGTCAATTATGGTAAATATAGCCGGCCCCCAGGGATCGCA8310.10331169299395049No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT8210.10206847165828321No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT8100.10070092818904922No Hit
CACTTGCACTGTCTCTGGTGACTCCATCCCCTGGAATGCTTACTACTGGG8050.10007931752121559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC12200.066.016844
ATCAACG12650.064.805353
CAACGCA12500.064.436455
AACGCAG12850.063.2488946
GTATCAA14150.060.0505561
TATCAAC14750.057.0414282
ACGCAGA14850.054.246187
CGCAGAG15300.053.1307268
GGTATCA5650.048.431361
GAATACG3000.045.5607729
GTGCTAG3250.044.3137131
TAGACGG15750.038.3550265
TACATGG22500.038.3526422
ATAGACG15950.037.4208764
AATAGAC16400.037.271053
GTACATG22950.037.0246351
TAATAGA17550.034.8287852
TATTGAG12700.032.843385
GCTAGAG4500.031.9605353
CATGGGG14800.031.5826194
Produced by FastQC (version 0.11.5)