FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819921
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA32010.3904034656997442No Hit
GTGTAGACTGGATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT26910.32820235120212804No Hit
GTGTATTACTGTGCGAGAGATTTTTCGCCAGTTGTCGGTGGGGCATATAT26590.3242995361748266No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT24880.30344386837268467No Hit
AGTCTACACAGTGGGGTCCCATCAAGATTCAGCGGCCGTGGATCTGGCAC23980.29246720110839947No Hit
ACTTATTACTGTCTACAAGATTCCAATTACCCTCGGACGTTCGGCCAGGG20040.24441379108475084No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT19290.23526656836451318No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA18520.22587541970506914No Hit
GTAATAAGTTGCGTAATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGA17230.21014219662626032No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15670.19111597336816596No Hit
GTCTACAAGATTCCAATTACCCTCGGACGTTCGGCCAGGGGACCGAGGTG15440.18831082506729308No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14090.17184582417086525No Hit
ATATATGCCCCACCGACAACTGGCGAAAAATCTCTCGCACAGTAATACAC14000.17074815744443672No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13510.16477197193388143No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT13320.16245467551142123No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13310.16233271254181805No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13050.15916167533213565No Hit
CTTGTAGACAGTAATAAGTTGCGTAATCTTCAGGCTGCAGGCCGCTGATG12750.15550278624404057No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT12080.14733126728062826No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11420.1392817112868191No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11380.13879385940840644No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10910.13306159983705745No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG10800.1317200071714226No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG10240.12489008087364513No Hit
GTAGGAGACAGGGTCACCATCACTTGCCGGGCAAGTCAGGACATTAGAAA9940.12123119178555009No Hit
GTATTACTGTGCGAGAGATTTTTCGCCAGTTGTCGGTGGGGCATATATCG9760.11903585833269302No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9630.11745033972785184No Hit
CCTTCCCAGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG9590.11696248784943916No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9580.116840524879836No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT9460.11537696924459795No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9130.11135219124769338No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG9130.11135219124769338No Hit
CTGTGAAGGGCCGCTTCACCATTTCCAGAGACAACGCCAAGAATTCACTG9060.1104984504604712No Hit
GTCTACACAGTGGGGTCCCATCAAGATTCAGCGGCCGTGGATCTGGCACA8880.10830311700761415No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8860.10805919106840782No Hit
CTCCTGTGTAGCCTCTGGATTCAGCTTTGATAGTTATTGGATGACCTGGG8860.10805919106840782No Hit
ACACAGTGGGGTCCCATCAAGATTCAGCGGCCGTGGATCTGGCACAGAAT8790.10720545028118562No Hit
GATCAGCACTGAGCGCAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG8730.10647367246356661No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8710.10622974652436028No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA8700.10610778355475711No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC8600.10488815385872542No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGG8410.1025708574362652No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT8330.10159515367943985No Hit
GGCATATATCGATGCTTTTGATGTCTGGGGCCAAGGGACAATGGTCACCG8300.10122926477063035No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAA8250.1006194499226145No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)