FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819921
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAACA25670.31307894297133504No Hit
GTGTAGACTGGATGCAGCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT24090.2938087937740343No Hit
GTGTATTACTGTGCGAGAGATTTTTCGCCAGTTGTCGGTGGGGCATATAT23690.2889302749899076No Hit
AGTCTACACAGTGGGGTCCCATCAAGATTCAGCGGCCGTGGATCTGGCAC20150.2457553837503857No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19950.2433161243583223No Hit
ACTTATTACTGTCTACAAGATTCCAATTACCCTCGGACGTTCGGCCAGGG17630.21502071541038711No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT15830.19306738088181666No Hit
GTAATAAGTTGCGTAATCTTCAGGCTGCAGGCCGCTGATGGTGAGAGTGA15690.1913598993073723No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA14890.18160286173911877No Hit
GTCTACAAGATTCCAATTACCCTCGGACGTTCGGCCAGGGGACCGAGGTG12840.15660045297046912No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12840.15660045297046912No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12640.15416119357840571No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12290.1498924896422948No Hit
ATATATGCCCCACCGACAACTGGCGAAAAATCTCTCGCACAGTAATACAC11570.14111115583086664No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11360.13854993346920008No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT10730.13086626638420043No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC10610.1294027107489624No Hit
CTTGTAGACAGTAATAAGTTGCGTAATCTTCAGGCTGCAGGCCGCTGATG10060.1226947474207881No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT9800.11952371021110571No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9440.11513304330539162No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT9240.11269378391332824No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8940.10903489482523315No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG8890.10842507997721731No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8780.10708348731158246No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG8750.10671759840277296No Hit
GTATTACTGTGCGAGAGATTTTTCGCCAGTTGTCGGTGGGGCATATATCG8470.10330263525388421No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8440.10293674634507471No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC14600.039.4433984
ATCAACG14700.039.1750763
TATCAAC15250.038.2342342
GTAATAC14100.037.311651
TAGGACC1553.6941856E-837.1531374
CAGCCGT14550.036.1157889
CAACGCA15950.036.1049275
ACAGCCG15250.035.8740968
GTATGTA9050.035.0384451
GAATACG1854.48199E-935.0193449
TACACGG8100.034.659066
GTGCTAG2302.910383E-1134.4671671
AATACAC15750.034.2781943
AACGCAG16600.034.257546
TATGTAC9350.033.1050342
CAGCGCT4900.032.319449
ACACGGG8550.031.992987
TAATACA16600.031.6557042
GTACACG8650.031.6231175
GTATCAA20700.031.3337881
Produced by FastQC (version 0.11.5)