FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867298
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCCTCGTCTCTAAGGCTGCTCATCTGCAGATACA16200.18678700977057483No Hit
GTTTATTACTGTGTTAGAGGTCAAACATCAGCAGACAACTGGTTCGGCCC16110.1857493041607383No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA15460.17825476364525225No Hit
GTGTATGATCCCACCGGTGCCAATAATGTATGAAATCCATTCCAGCCCCT14710.16960721689661454No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT14320.1651104925873229No Hit
ATCATACACTACGCAGACTCTGTGAAGGGCCGATTCACCATTTCCAGAGA13380.15427223399569698No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13330.15369573087912114No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT12590.1451634847537986No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT12210.14078206106782212No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11890.13709244112173669No Hit
GTATTACTGTCAGCAGCGTAAGATCTGGCCTCCGATCACCTTCGGCCAAG11740.13536293177200917No Hit
GAGTTAGGGCTGTGCTGGATTTTCCTTGTTGCTATTTTAGAAGGTGTCCA11070.12763779000989278No Hit
GCCTTAGAGACGAGGACACGGCTGTTTATTACTGTGTTAGAGGTCAAACA10600.12221866071407983No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT9830.11334051271881175No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG9460.10907438965615049No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT9330.10757548155305328No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA9040.10423176347691336No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8980.10353995973702233Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTAAGGCTGCTCATCTGCAGATACAGAGAGTTTTTGGCATTGTCTCTG8770.10111864664740379No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)