FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847292
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATACTTTGGGTGCGACAGGCCCCTGGACAAGGCCTTGAGTGGATG45460.5365328599821549No Hit
GTAATATGCGGCCGTGTCGTCAGATGTCAGCCTGCTCAACTCCATGTAGG27270.32184890214943607No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC27190.32090471761801126No Hit
CCATAGTACTGCCGCTTTTATAGGAAGCGCAATAATATTCAGCCTCGTCC19890.23474787912549627No Hit
GACCTAGGACGGTCACCGTGGTCCCTCCGCCGAATACCATAGTACTGCCG18680.22046708808769588No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA18440.2176345344934214No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT18210.21492000396557503No Hit
GTACTATGGTATTCGGCGGAGGGACCACGGTGACCGTCCTAGGTCAGCCC17180.20276362812348048No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT16850.19886886693135306No Hit
GCCTACATGGAGTTGAGCAGGCTGACATCTGACGACACGGCCGCATATTA16460.19426596734065704No Hit
GTATATAATAGTCGGTGAAGGTGTATCCAGAAGCCTTGCAGGAGACCTTC16330.19273166747709172No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT15900.1876566756206833No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT15070.1778607611071508No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14270.16841891579290255No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT13490.15921311661151055No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC13380.15791486288080142No Hit
GCCTTGGGCTGACCTAGGACGGTCACCGTGGTCCCTCCGCCGAATACCAT13350.15756079368151713No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13150.15520033235295505No Hit
GTCTGGGGCTGAGGTGAGGGAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA12850.15165964036011198No Hit
GCATATTACTGTGCGAGAGATCTTAAAGCGACTTCAGTAACTACGAATGG12780.15083347889511525No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12750.15047940969583096No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGAGGCTGCTATCT12580.1484730175665532No Hit
CTATTATATACTTTGGGTGCGACAGGCCCCTGGACAAGGCCTTGAGTGGA12570.1483549945001251No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT12320.14540441783942254No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG12270.14481430250728203No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTTATTATAACTTTGTCGCCT11950.14103756438158274No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG11700.13808698772088018No Hit
TTTATAGGAAGCGCAATAATATTCAGCCTCGTCCTCAGCCAGGAGCCCAG11540.1361986186580305No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11410.1346643187944652No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG11280.13313001893089985No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC11270.13301199586447174No Hit
TCCTATAAAAGCGGCAGTACTATGGTATTCGGCGGAGGGACCACGGTGAC11010.12994339613734107No Hit
CCATGTAGGCTGTGCTGGTGGACGTGTCCCTGGTCATGGTGACCCGGCCC10900.12864514240663194No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10630.12545851961307317No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA10540.12439631201522025No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA10320.121799804553802No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA10320.121799804553802No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10200.12038352775666478No Hit
ACCTAGGACGGTCACCGTGGTCCCTCCGCCGAATACCATAGTACTGCCGC10010.11814108949453081No Hit
GTCCTCTAGAGAACCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACC9980.11778702029524651No Hit
ATAATATTCAGCCTCGTCCTCAGCCAGGAGCCCAGAGATGGCCAGGGAGG9940.11731492802953408No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGAGGCTGCTATCTGCCTTCCAG9930.11719690496310597No Hit
CCCTAACAGTGGTGGCGCAAACTATCCACAGAAGTTTCAGGGCCGGGTCA9870.11648876656453738No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9710.11460039750168774No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA9610.1134201668374067No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT9580.1130660976381224No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9570.11294807457169428No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA9440.11141377470812895No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG9430.11129575164170084No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC9410.11105970550884464No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT9150.10799110578171398No Hit
CCCCTGGACAAGGCCTTGAGTGGATGGGATGGATGAACCCTAACAGTGGT9070.10704692125028915No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA8950.10563064445315191No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8930.10539459832029571No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC8880.1048044829881552No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT8840.1043323907224428No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG8800.10386029845673038No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA8770.10350622925744607No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC8730.10303413699173367No Hit
CTAGAGAACCCCCTGAGAGCTCCGTTCCTCACCATGGACTGGACCTGGAG8590.10138181406174024No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5900.067.2705461
GTATCAA17200.065.869671
TCAACGC18100.062.4429324
ATCAACG18200.062.0998343
TATCAAC18100.061.705742
CAACGCA18350.061.5922055
AACGCAG19150.059.019176
CGCAGAG20950.053.261078
GCAGAGT23600.046.9754459
ACGCAGA25800.043.5278367
TATTGAG8900.042.0606045
TATATAC19700.035.446043
ATATACT19150.035.336324
TTATTGA11050.034.5283474
TATACTT19450.034.421165
ATTGAGT7600.034.0997436
GTACATG19800.033.5302621
GTACACG8200.033.3604555
TTATATA20600.032.8800962
GGTTTAT7800.032.380811
Produced by FastQC (version 0.11.5)