FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847292
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATACTTTGGGTGCGACAGGCCCCTGGACAAGGCCTTGAGTGGATG41840.49380850993518177No Hit
GTAATATGCGGCCGTGTCGTCAGATGTCAGCCTGCTCAACTCCATGTAGG25920.3059157881816422No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC23110.2727513065153454No Hit
GTATATAATAGTCGGTGAAGGTGTATCCAGAAGCCTTGCAGGAGACCTTC19420.22920079500337548No Hit
CCATAGTACTGCCGCTTTTATAGGAAGCGCAATAATATTCAGCCTCGTCC17210.20311769732276477No Hit
GACCTAGGACGGTCACCGTGGTCCCTCCGCCGAATACCATAGTACTGCCG16400.19355782894208845No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT16070.18966306774996106No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA15890.18753865255425523No Hit
GTACTATGGTATTCGGCGGAGGGACCACGGTGACCGTCCTAGGTCAGCCC15460.18246366069784678No Hit
GCCTACATGGAGTTGAGCAGGCTGACATCTGACGACACGGCCGCATATTA14540.17160553858646135No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT14480.1708974001878927No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13010.15354800942296162No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT12930.15260382489153682No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT12270.14481430250728203No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC12180.1437520949094291No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11920.14068349518229842No Hit
GCCTTGGGCTGACCTAGGACGGTCACCGTGGTCCCTCCGCCGAATACCAT11780.139031172252305No Hit
GTCTGGGGCTGAGGTGAGGGAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA11420.13478234186089327No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11350.13395618039589657No Hit
GCATATTACTGTGCGAGAGATCTTAAAGCGACTTCAGTAACTACGAATGG11000.129825373070913No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGAGGCTGCTATCT10730.12663875027735422No Hit
TCCTATAAAAGCGGCAGTACTATGGTATTCGGCGGAGGGACCACGGTGAC10590.12498642734736078No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG10590.12498642734736078No Hit
CTATTATATACTTTGGGTGCGACAGGCCCCTGGACAAGGCCTTGAGTGGA10310.12168178148737388No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT10100.11920329709238374No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA10070.11884922789309944No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT10060.11873120482667132No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9810.11578062816596875No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTTATTATAACTTTGTCGCCT9800.11566260509954064No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC9670.11412830523597531No Hit
TTTATAGGAAGCGCAATAATATTCAGCCTCGTCCTCAGCCAGGAGCCCAG9650.1138922591031191No Hit
CCATGTAGGCTGTGCTGGTGGACGTGTCCCTGGTCATGGTGACCCGGCCC9400.11094168244241655No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9400.11094168244241655No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG9130.10775505964885777No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9080.10716494431671725No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9040.10669285205100484No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA9030.10657482898457675No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG9030.10657482898457675No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA8930.10539459832029571No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA8540.10079169872959971No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA8530.1006736756631716No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)