FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822881
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCTGCGGCGGTCACTGAGGTCATCTTCAGGGAGA31460.38231530440002864No Hit
GTCTCTGGTGGCTCCATCAACAGCAATGATTACCACTGGGACTGGATCCG26390.32070250740994144No Hit
GTATATTACTGTGCGAGACATAATCACGGGTGTGACTACTGGGGCCAGGG21030.25556550704172293No Hit
CTATAACCCGTCCCTCGAGAGTCGAGCCACCATATCCATCGACATGTCCA18940.23016693786829445No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT16970.20622665974788576No Hit
ATCTATTATCTTGGAGGCTCCCTCTATAACCCGTCCCTCGAGAGTCGAGC13990.17001243193122698No Hit
ATATACAGCCGTGTCTGCGGCGGTCACTGAGGTCATCTTCAGGGAGAACT13510.16417926771914773No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12090.14692282359174658No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11700.14218337766943215No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11520.13999594108990243No Hit
ATTTACGACTGGGTAGCCTGGTATCAGCAAAAACCAGGCAAAGCCCCTAA11220.1363502134573529No Hit
GCTGTATATTACTGTGCGAGACATAATCACGGGTGTGACTACTGGGGCCA10790.1311246705173652No Hit
GTAGGAGACAGAGTCATCATCACTTGTCGGGCCAGTCACGATATTTACGA10650.12942333095550876No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9310.11313908086345414No Hit
ATATTACTGTGCGAGACATAATCACGGGTGTGACTACTGGGGCCAGGGAA8650.10511848007184513No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8500.10329561625557038No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8460.10280951923789709No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8400.10208037371138719No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG8380.10183732520255054No Hit
CAGTAATATACAGCCGTGTCTGCGGCGGTCACTGAGGTCATCTTCAGGGA8290.1007436069127857No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG406.820243E-572.15831
GTATCAA22800.060.4484061
TCAACGC24800.055.73214
ATCAACG24700.055.6662943
TATACGG400.006206335554.026592
CAACGCA25450.054.0258335
TATCAAC25450.053.778922
AACGCAG26150.052.579636
ACGCAGA28450.048.075887
CGCAGAG29400.046.5224088
GAATACG3150.038.8502929
ACCGATC750.001535869238.3932278
TACGGGT1102.5393887E-432.72154
GTGCTAG4650.032.587621
GCAGAGT44400.030.8053769
CAGCCGT15300.027.28939
GCTAGAG5050.027.084333
ACAGCCG16200.026.6619638
TATACAG18400.026.6040025
CTTATAG2456.849223E-826.4620022
Produced by FastQC (version 0.11.5)