FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822881
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCTGCGGCGGTCACTGAGGTCATCTTCAGGGAGA25990.3158415372332087No Hit
GTCTCTGGTGGCTCCATCAACAGCAATGATTACCACTGGGACTGGATCCG24060.29238735613047334No Hit
GTATATTACTGTGCGAGACATAATCACGGGTGTGACTACTGGGGCCAGGG19240.23381266550084398No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT14130.17171377149308345No Hit
CTATAACCCGTCCCTCGAGAGTCGAGCCACCATATCCATCGACATGTCCA13740.16697432557076905No Hit
ATCTATTATCTTGGAGGCTCCCTCTATAACCCGTCCCTCGAGAGTCGAGC11660.14169728065175888No Hit
ATATACAGCCGTGTCTGCGGCGGTCACTGAGGTCATCTTCAGGGAGAACT11120.1351349709131697No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9910.12043053612855323No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT9610.11678480849600369No Hit
GCTGTATATTACTGTGCGAGACATAATCACGGGTGTGACTACTGGGGCCA9310.11313908086345414No Hit
ATTTACGACTGGGTAGCCTGGTATCAGCAAAAACCAGGCAAAGCCCCTAA9270.11265298384578086No Hit
GTAGGAGACAGAGTCATCATCACTTGTCGGGCCAGTCACGATATTTACGA8450.10268799498347878No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC15850.043.1431544
ATCAACG15950.042.4213753
GAATACG2750.039.2623379
TATCAAC17800.039.2255742
CAACGCA17300.038.694965
GTATCAA19300.038.1019441
AACGCAG18050.037.087146
ACGCAGA18850.035.513157
TACACCG1459.1986476E-734.7494245
GTGCTAG4050.033.8222771
CGCAGAG19500.033.591118
ATACCGT1153.2976718E-431.2960646
GTAATAT16050.030.544861
TATACAG16850.030.330255
CAGCCGT14350.030.0965639
AATATAC16600.029.9197923
GTACATG26950.028.8914431
ACAGCCG15150.028.5073058
TACATGG26850.028.417062
TAATATA17400.028.1304872
Produced by FastQC (version 0.11.5)