FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688540
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATATACAGCCGTGTCCTCGACTCTCAGAGTGTTCATTTGCAGATACA33440.485665320823772No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG24760.35960147558602257No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24540.3564063090016557No Hit
GTATATTATTGTGCAAGAGATTTAAGGGGATCCAACCACTGGGGCCAGGG20540.2983123711040753No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC18000.26142272053911175No Hit
ATATACAGCCGTGTCCTCGACTCTCAGAGTGTTCATTTGCAGATACAGCG17910.26011560693641617No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT17530.25459668283614606No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT17190.24965869811485172No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG16700.24254219072239813No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG14330.20812153251808177No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTCCTG13660.19839079792023703No Hit
GCTGTATATTATTGTGCAAGAGATTTAAGGGGATCCAACCACTGGGGCCA12870.1869172451854649No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC12290.17849362419031575No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT11660.16934382897144684No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA11380.1652772533186162No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10930.1587416853051384No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC10110.14683242803613444No Hit
GTAATAATCAGCCTCGTCCTCAGGCTGGAGCCCAGAGATGGTCAGGGAGG10100.14668719319139048No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG10050.14596101896767072No Hit
GATTAAGACTGATGGGAGTGTGACAACCTACGCGGACTCCGTGAAGGGCC10030.14567054927818282No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9880.14349202660702357No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT9620.13971592064368082No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9180.133325587474947No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC8940.12983995120109218No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCCGGGGGAGGCT8680.12606384523774944No Hit
GTGTGAGGTGCAGCTGGTGGAGTCCGGGGGAGGCTTAGTTGAGCCTGGGG8590.12475673163505388No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT8430.12243297411915068No Hit
CTCTTGTGCAGCCTCTGGATTCACCTTCAGTGACTACTGGATGCACTGGA8370.12156156505068696No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG8330.12098062567171117No Hit
CTCCTGCACTGGAACCAGCAGGGACGTTGGTGGTTATAACTATGTCTCCT8190.11894733784529583No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC8140.11822116362157609No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC8050.11691405001888053No Hit
TCTCTGGGCTCCAGCCTGAGGACGAGGCTGATTATTACTGCAGCTCATCT8020.11647834548464868No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTCCTGT7990.11604264095041682No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC7940.11531646672669707No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT7930.11517123188195312No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTG7920.11502599703720917No Hit
GGTTATAACTATGTCTCCTGGTATCAACAACACCCAGGCAAAGGCCCCAA7900.11473552734772127No Hit
CCCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGGGACGTT7880.11444505765823337No Hit
GATTATTACTGCAGCTCATCTACAAACAGGAACACTTGGGTGTTCGGCGG7820.11357364858976966No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC7810.11342841374502571No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCT7760.11270223952130594No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7580.11008801231591483No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT7450.10819995933424348No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA7390.10732855026577978No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA7250.10529526243936445No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC7200.1045690882156447No Hit
GTCTTAATCTGTGAGACCCACACCGGCCCCTTCCCTGGAGGTTGGCGGAT7120.10340720945769309No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7050.10239056554448543No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT6930.10064774740755802No Hit
CTCTGAGAGTCGAGGACACGGCTGTATATTATTGTGCAAGAGATTTAAGG6920.10050251256281408No Hit
GTGTAGAGAACATATATGCATAAACATAGGTCAATTATATGTCTCCATTA6900.10021204287332618No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG18050.053.4461863
TCAACGC18150.052.7550624
CAACGCA18750.051.06695
AACGCAG19300.049.984656
ACGCAGA21650.044.559067
CGCAGAG21750.044.0231938
GCAGAGT23450.040.8317459
CAGAGCG10450.038.5798769
GTCATAG11050.038.5263371
ATAGGTG11150.038.094854
TAGGTGG11400.037.2594385
ACAGCCG14400.034.9965068
TATACAG15650.034.5013435
CAGCCGT14200.034.475439
AATATAC14600.034.0243
ACAGAGC12300.033.947838
TAATATA15400.033.6883352
TACAGCC15500.033.4418227
GTGGTCA13100.032.9738048
GTATCAA30100.032.601711
Produced by FastQC (version 0.11.5)