FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688540
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATATACAGCCGTGTCCTCGACTCTCAGAGTGTTCATTTGCAGATACA28010.4068028001278067No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG23640.3433351729747001No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG19760.28698405321404713No Hit
GTATATTATTGTGCAAGAGATTTAAGGGGATCCAACCACTGGGGCCAGGG19380.28146512911377697No Hit
ATATACAGCCGTGTCCTCGACTCTCAGAGTGTTCATTTGCAGATACAGCG15870.2304876986086502No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC15670.2275830017137712No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG15010.21799750196067041No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT14120.2050716007784588No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13110.19040288145931972No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC12090.17558892729543674No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTCCTG11410.16571295785284806No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG10950.1590321549946263No Hit
GCTGTATATTATTGTGCAAGAGATTTAAGGGGATCCAACCACTGGGGCCA10550.15322276120486827No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA10190.14799430679408604No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT9730.14131350393586428No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9490.13782786766200947No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC9360.1359398146803381No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG9350.13579457983559418No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT8830.1282423679089087No Hit
GATTAAGACTGATGGGAGTGTGACAACCTACGCGGACTCCGTGAAGGGCC8700.12635431492723734No Hit
GTAATAATCAGCCTCGTCCTCAGGCTGGAGCCCAGAGATGGTCAGGGAGG8420.12228773927440671No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7910.11488076219246521No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC7630.1108141865396346No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA7560.10979754262642692No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG7540.10950707293693902No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC7460.10834519417898741No Hit
GGTTATAACTATGTCTCCTGGTATCAACAACACCCAGGCAAAGGCCCCAA7330.10645714119731604No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA7190.10442385337090074No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCCGGGGGAGGCT7170.10413338368141284No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC7070.10268103523397333No Hit
GATTATTACTGCAGCTCATCTACAAACAGGAACACTTGGGTGTTCGGCGG6930.10064774740755802No Hit
GTGTGAGGTGCAGCTGGTGGAGTCCGGGGGAGGCTTAGTTGAGCCTGGGG6910.10035727771807013No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATACG2400.050.990599
TCAACGC9400.049.778044
ATCAACG9500.049.2540663
GTGCTAG2750.047.1562461
CAACGCA10500.045.248795
AACGCAG11150.042.6109666
GTCATAG12100.042.2739031
ATAGGTG11300.042.0453384
TAGGTGG11800.041.4838685
ACGCAGA12050.038.8310057
CGCAGAG12250.038.7846768
GTGGTCA13000.038.2083328
GTCTAGC1001.443558E-436.0221331
GCTAGAG3600.035.9933553
TAATATA15950.035.6548582
AGGTGGT14550.035.1275376
AATATAC15900.034.4087453
ATAATAT17250.033.4118351
ACAGCCG16400.032.9207538
TACAGCC16900.032.798687
Produced by FastQC (version 0.11.5)