FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062643
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTTAATTCCAGAAACA50020.47071311814033495No Hit
GTCTATTACTGTGCGACAGGGGGCTATGGGTCGGGGAGCTCGGCCTACTG25020.2354506640518029No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19520.18369292415232585No Hit
TCCCAATACTACGCAGACTCAGTGAAGGGCCGCTTCATCATCTCCAGAGA15400.14492167171853576No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14950.14068694754494218No Hit
GTATTGGGAACTAGTAGGACTAGTAATAGATGAGACCCACTCCAGCCCCT13980.13155876432630714No Hit
GTATAAGCACTGACACTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGA13110.12337163092402623No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12700.1195133266769743No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAAAGCCACCCTCTCCT12330.11603144235646401No Hit
CTATTACTGTCAGCAGTATGGTAGCTCATCCCCGTACAGTTTTGGCCAGG11240.10577399935820403No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC10650.10022180544171466No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)