FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058310
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACATAGCCGAGTCTTCAGGTTTAAGGCTGTCCATTTGCAAGTACA51920.4905934933998545No Hit
ATGTATTACTGTGCGAGAGACTGGAGGGGGCATGATGCTTTCGATACTTG42320.3998828320624392No Hit
ATATTACTGTCAGCACTATGGTACCTCACGCTTTCCTTTCGGCCCTGGGA25550.2414226455386418No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGAACCTGGTGGAGTCTGGGGGAGGCG23210.21931192183764683No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA20290.1917207623475163No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20250.19134280125861042No Hit
GCTCTACACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTCGC19620.18538991410834255No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19040.17990947831920703No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC18130.17131086354659789No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16810.1588381476127033No Hit
CAGTAATATACTGCAAAATCGTCAGGCTCCAGTCTGCTGATGGTGAGAGT16390.15486955617919135No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16360.15458608536251192No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15670.1480662565788852No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14900.1407905056174467No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14870.14050703480076726No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT14360.13568803091721707No Hit
GTATTACTGTGCGAGAGACTGGAGGGGGCATGATGCTTTCGATACTTGGG14270.1348376184671788No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14210.13427067683381996No Hit
CAGTAATACATAGCCGAGTCTTCAGGTTTAAGGCTGTCCATTTGCAAGTA13960.13190842002815809No Hit
GTGTAGAGCATAGGTTCCGAAGGTACGCCCAGAATCTGCACAGGAGAGTT13350.12614451342234317No Hit
GTATATTACTGTCAGCACTATGGTACCTCACGCTTTCCTTTCGGCCCTGG12950.12236490253328419No Hit
GTAATATACTGCAAAATCGTCAGGCTCCAGTCTGCTGATGGTGAGAGTGA12260.11584507374965748No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11890.11234893367727793No Hit
GCCTTAAACCTGAAGACTCGGCTATGTATTACTGTGCGAGAGACTGGAGG11830.11178199204391907No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11760.11112056013833375No Hit
GGTTTAAGGCTGTCCATTTGCAAGTACATCGTGCTCTTGGAATTGTCTCT11730.11083708932165433No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11150.10535665353251882No Hit
GGGCTGGAGTGGGTCGCAGTTATCTCTTTTCATGGAGTTCAAACTGACTA11040.1043172605380276No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG10950.10346684808798935No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10900.10299439672685697No Hit
GTTAGGACCCAGAGGGAACCATGGACACCCCAGCGCAGCTTCTCTTCCTC10720.10129357182678043No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG10630.10044315937674216No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18950.071.1206361
TCAACGC20200.066.002914
ATCAACG20450.065.1960143
CAACGCA21050.063.3376965
TATCAAC22400.060.1639372
AACGCAG22800.058.4734886
GGTATCA7350.055.892131
CGCAGAG26000.051.22838
GCAGAGT29450.045.715969
ACGCAGA32850.040.790217
GTACATG27650.037.5344921
TACATGG28200.036.0340162
ACATGGG28150.035.8420033
ATAGCCG21450.035.5787058
TAGCCGA21400.035.3253979
GTAATAC22150.034.490171
TACGGGT850.00282357633.9143684
GTATTAC20900.033.103023
CTGTGCG21250.032.86439
TACATAG24350.032.2604335
Produced by FastQC (version 0.11.5)