FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058310
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACATAGCCGAGTCTTCAGGTTTAAGGCTGTCCATTTGCAAGTACA48190.4553486218593796No Hit
ATGTATTACTGTGCGAGAGACTGGAGGGGGCATGATGCTTTCGATACTTG40820.38570929122846803No Hit
ATATTACTGTCAGCACTATGGTACCTCACGCTTTCCTTTCGGCCCTGGGA24570.23216259886044732No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGAACCTGGTGGAGTCTGGGGGAGGCG22420.21184719033175534No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21240.20069733820903138No Hit
GCTCTACACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTCGC20560.19427199969763112No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA18700.1766968090635069No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT18370.17357863008003327No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC16690.15770426434598558No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16480.15571996862922963No Hit
CAGTAATATACTGCAAAATCGTCAGGCTCCAGTCTGCTGATGGTGAGAGT15170.1433417429675615No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14920.14097948616189962No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14850.1403180542563143No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT14780.13965662235072898No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14630.13823926826733188No Hit
GTATTACTGTGCGAGAGACTGGAGGGGGCATGATGCTTTCGATACTTGGG14410.13616048227834945No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14290.13502659901163175No Hit
CAGTAATACATAGCCGAGTCTTCAGGTTTAAGGCTGTCCATTTGCAAGTA14040.1326643422059699No Hit
GTGTAGAGCATAGGTTCCGAAGGTACGCCCAGAATCTGCACAGGAGAGTT13370.1263334939667961No Hit
GTATATTACTGTCAGCACTATGGTACCTCACGCTTTCCTTTCGGCCCTGG12960.12245939280551067No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12670.11971917491094292No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12440.11754589864973401No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11780.11130954068278671No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA11720.11074259904942786No Hit
GTAATATACTGCAAAATCGTCAGGCTCCAGTCTGCTGATGGTGAGAGTGA11150.10535665353251882No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10670.10082112046564805No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13250.055.5329971
TCAACGC14200.051.29034
ATCAACG14350.050.754163
CAACGCA15500.046.5255055
AACGCAG15950.045.1551136
TATCAAC16500.043.7037052
CGCAGAG18700.038.1313138
CAGCGCT6800.037.072119
GGTATCA7250.036.8154831
GTACATG28300.034.157431
ACATGGG26450.034.078983
TAGCCGA18500.033.482279
TACATGG28350.033.3211862
GCAGAGT21250.033.216619
ATAGCCG19200.033.0118338
GGCAGCG8000.032.4116137
TACATAG21000.031.249635
CATGGGA14300.030.7606854
GTAATAC19550.030.6265561
CTGTGCG19600.030.1332479
Produced by FastQC (version 0.11.5)