FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1105444
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCCTCGCCTCTCAGGCCGTTCATTTGAAGATACA31900.2885718317707636No Hit
GTTTATTACTGTGCGAGAGGCCCTAGGGGATCGCTCTACGGTATGGACGT22050.19946736333997925No Hit
GTTCCATACCGTACAGTTTTGGCCAGGGGACCCACCTGGAGATCAAACGA17650.1596643520612532No Hit
GTATGGAACCATATTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCC17420.15758374010804707No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT15210.13759177307941423No Hit
GTATTACTGTCAGCAATATGGTTCCATACCGTACAGTTTTGGCCAGGGGA13940.1261031766421456No Hit
ATGTATAACCACCGGTGAAAAGAATTGAGACCCACTCCAGTTCCTTCCCT11220.10149767876075134No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC14350.058.2768174
GTATCAA15400.056.6774861
ATCAACG15000.055.7514883
CGCAGAG17700.048.0245978
CAACGCA17500.047.7978325
TATCAAC18550.045.4707072
AACGCAG19000.043.976436
GCAGAGT24100.036.16799
TACATGG29550.031.9597632
CAGCCGT15300.030.1330819
ACAGCCG15350.030.0349278
ACGCAGA32600.027.1795657
ACCGTAC12350.026.248268
ACATGGG37100.025.4558243
CTGTGCG18300.024.7995859
TAAACAG21200.024.4897825
GTACATG42500.024.4410271
CCGTACA14000.024.1838159
AACAGCC22050.023.1955537
GTAATAA21300.023.0290051
Produced by FastQC (version 0.11.5)