FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2358517
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36510.15480066499414674No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT26270.11138355161315351No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47550.071.502751
TCAACGC53300.063.225664
ATCAACG54300.062.193893
GGTATCA21600.062.028381
CAACGCA55950.060.22855
AACGCAG58650.057.45346
TATCAAC60350.056.3169862
CGCAGAG72650.046.579088
GCAGAGT84500.040.132199
ACGCAGA93450.036.211567
GTACATG63650.032.8194161
ACATGGG67300.030.707293
TACATGG66750.030.4209332
CATGGGG46700.024.0537324
TACACGG14000.023.1431436
GTACACG14150.022.898785
GTATAGG8450.021.3115251
ACGGGGT16000.021.1498139
GAATACG3758.42374E-921.1198129
ACCGTAT2053.8442147E-421.0729858
Produced by FastQC (version 0.11.5)